Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA.
Pediatr Res. 2010 Apr;67(4):382-6. doi: 10.1203/PDR.0b013e3181d00b73.
Epidermal innate immunity is a complex process involving a balance of pro- and anti-inflammatory cytokines, structural proteins, and specific antigen presenting cells occurring against a background of neuroendocrine modulators such as cortisol. In this study, a multiplex array system was used to simultaneously determine multiple molecular factors critical for development of epidermal innate immune function from the skin surface of premature and term infants, healthy adults, and vernix caseosa. Samples were analyzed for Keratin 1,10,11, Keratin 6, involucrin, albumin, fibronectin and cortisol, and cytokines IL-1, TNFalpha, IL-6, IL-8, MCP1, IP10, IFNgamma, and IL-1 receptor antagonist. Keratin 1,10,11 was decreased and involucrin was increased in infants versus adults. All infants had elevated IL1alpha and reduced TNFalpha versus adults. IL-6, IL-8, and MCP1 were significantly increased in premature versus term infants and adults. Skin surface cortisol and albumin were significantly elevated in premature infants. The biomarker profile in premature infants was unique with differences in structural proteins, albumin, and cytokines IL-6, IL-1beta, IL-8, and MCP1. The higher infant IL1alpha may be associated with skin barrier maturation. The significant elevations in skin surface cortisol for preterm infants may reflect a neuroendocrine response to the stress of premature birth.
表皮先天免疫是一个复杂的过程，涉及到促炎和抗炎细胞因子、结构蛋白和特定抗原呈递细胞的平衡，而这些过程发生在神经内分泌调节剂（如皮质醇）的背景下。在这项研究中，使用多重阵列系统同时从早产儿和足月儿、健康成年人和胎脂的皮肤表面确定对表皮先天免疫功能发展至关重要的多种分子因素。对角蛋白 1、10、11、角蛋白 6、板层素、白蛋白、纤维连接蛋白和皮质醇以及细胞因子 IL-1、TNFalpha、IL-6、IL-8、MCP1、IP10、IFNgamma 和 IL-1 受体拮抗剂进行了分析。与成年人相比，婴儿的角蛋白 1、10、11 减少，板层素增加。所有婴儿的白细胞介素 1α升高，肿瘤坏死因子α降低。与足月儿和成年人相比，早产儿的 IL-6、IL-8 和 MCP1 显著增加。早产儿的皮肤表面皮质醇和白蛋白显著升高。早产儿的生物标志物谱具有独特性，其差异在于结构蛋白、白蛋白以及细胞因子 IL-6、IL-1β、IL-8 和 MCP1。较高的婴儿白细胞介素 1α可能与皮肤屏障成熟有关。早产儿皮肤表面皮质醇的显著升高可能反映了对早产应激的神经内分泌反应。
