姜黄素对 CCRF-CEM 白血病细胞的细胞毒性及其对 DNA 损伤的影响。
Cytotoxic activity of curcumin towards CCRF-CEM leukemia cells and its effect on DNA damage.
机构信息
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
出版信息
Molecules. 2009 Dec 17;14(12):5328-38. doi: 10.3390/molecules14125328.
The cytotoxic activity of curcumin towards CCRF-CEM human T-cell leukemia cells was measured by the MTT assay. Tumor cells were more sensitive to the cytotoxic activity of curcumin or curcumin-Cu (II)compared to normal cells, and the IC(50) of curcumin towards CCRF-CEM cells was 8.68 microM, and that of curcumin-Cu (II) was 8.14 microM. The cell cycle distribution of curcumin-treated CCRF-CEM cells was analyzed by flow cytometry. DNA damage induced by oxidants such as curcumin-Cu (II) ions is considered as one of the main causes of cell inactivation. Therefore, we analyzed the effect of curcumin on DNA damage by agarose gel electrophoresis and atomic force microscopy (AFM). Gel electrophoresis analyses showed that curcumin or Cu (II) alone failed to cause DNA damage in pBR322 plasmid DNA as compared with the normal plasmid. However, DNA plasmids were mostly damaged after treatment with curcumin of different concentrations in the presence of Cu (II). Two forms were observed by means of AFM: closed circular plasmids and linear plasmids. DNA damage induced by a combination of curcumin and Cu (II) was also found by agarose gel electrophoresis, which was applied as control method to verify the results obtained by AFM.
姜黄素对 CCRF-CEM 人 T 细胞白血病细胞的细胞毒性活性通过 MTT 检测法来测量。肿瘤细胞比正常细胞对姜黄素或姜黄素-Cu(II)的细胞毒性活性更敏感，姜黄素对 CCRF-CEM 细胞的 IC50 为 8.68μM，而姜黄素-Cu(II)的 IC50 为 8.14μM。用流式细胞术分析姜黄素处理的 CCRF-CEM 细胞的细胞周期分布。氧化剂（如姜黄素-Cu(II)离子）引起的 DNA 损伤被认为是细胞失活的主要原因之一。因此，我们通过琼脂糖凝胶电泳和原子力显微镜（AFM）分析了姜黄素对 DNA 损伤的影响。凝胶电泳分析表明，与正常质粒相比，姜黄素或 Cu(II)单独处理不能引起 pBR322 质粒 DNA 的 DNA 损伤。然而，在存在 Cu(II)的情况下，用不同浓度的姜黄素处理后，DNA 质粒大部分受损。通过 AFM 观察到两种形式：闭合的圆形质粒和线性质粒。通过琼脂糖凝胶电泳也发现了姜黄素和 Cu(II)联合诱导的 DNA 损伤，该方法被用作控制方法来验证 AFM 获得的结果。
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