Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269, USA.
Mamm Genome. 2010 Feb;21(1-2):77-87. doi: 10.1007/s00335-009-9242-1. Epub 2009 Dec 22.
Multiple Genome Rearrangement (MGR) analysis was used to define the trajectory and pattern of chromosome rearrangement within muroid rodents. MGR was applied using 107 chromosome homologies between Mus, Rattus, Peromyscus, the muroid sister taxon Cricetulus griseus, and Sciurus carolinensis as a non-Muroidea outgroup, with specific attention paid to breakpoint reuse and centromere evolution. This analysis revealed a high level of chromosome breakpoint conservation between Rattus and Peromyscus and indicated that the chromosomes of Mus are highly derived. This analysis identified several conserved evolutionary breakpoints that have been reused multiple times during karyotypic evolution in rodents. Our data demonstrate a high level of reuse of breakpoints among muroid rodents, further supporting the "Fragile Breakage Model" of chromosome evolution. We provide the first analysis of rodent centromeres with respect to evolutionary breakpoints. By analyzing closely related rodent species we were able to clarify muroid rodent karyotypic evolution. We were also able to derive several high-resolution ancestral karyotypes and identify rearrangements specific to various stages of Muroidea evolution. These data were useful in further characterizing lineage-specific modes of chromosome evolution.
多重基因组重排 (MGR) 分析用于定义鼠形亚目啮齿动物中染色体重排的轨迹和模式。MGR 应用于 Mus、Rattus、Peromyscus、鼠形亚目姐妹分类群 Cricetulus griseus 和 Sciurus carolinensis 之间的 107 条染色体同源性，其中 Sciurus carolinensis 被用作非鼠形亚目外群，特别关注断点的重复使用和着丝粒的进化。该分析揭示了 Rattus 和 Peromyscus 之间染色体断点的高度保守性，并表明 Mus 染色体高度衍生。该分析确定了几个在啮齿动物的核型进化中多次重复使用的保守进化断点。我们的数据表明，鼠形啮齿动物中存在高水平的断点重复使用，进一步支持了染色体进化的“脆弱断裂模型”。我们首次对啮齿动物的着丝粒进行了关于进化断点的分析。通过分析密切相关的啮齿动物物种，我们能够阐明鼠形啮齿动物的核型进化。我们还能够推导出几个高分辨率的祖先核型，并确定特定于各种 Muroidea 进化阶段的重排。这些数据有助于进一步描述谱系特异性的染色体进化模式。
