从实体组织中快速无柱富集单核细胞
Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues.
作者信息
Scoville Steven D, Keller Karen A, Cheng Stephanie, Zhang Michael, Zhang Xiaoli, Caligiuri Michael A, Freud Aharon G
机构信息
1] Medical Scientist Training Program, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA [2] Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, Ohio 43210, USA [3] The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
出版信息
Sci Rep. 2015 Jul 30;5:12490. doi: 10.1038/srep12490.
We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.
我们开发了一种快速阴性选择方法，用于从人体组织中富集稀有单核细胞。在Ficoll分离步骤中，不需要的细胞和与抗体结合的细胞会被选择性去除，且无需基于磁性的试剂和设备。这种新方法快速、可定制、成本低、效率高且易于操作，每个样本的总成本不到基于磁珠柱方法成本的十分之一。
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