Bryceson Yenan T, Fauriat Cyril, Nunes João M, Wood Stephanie M, Björkström Niklas K, Long Eric O, Ljunggren Hans-Gustaf
Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.
Methods Mol Biol. 2010;612:335-52. doi: 10.1007/978-1-60761-362-6_23.
Natural killer (NK) cells are a subset of lymphocytes that contribute to innate immunity through cytokine secretion and target cell lysis. NK cell function is regulated by a multiplicity of activating and inhibitory receptors. The advance in instrumentation for multi-color flow cytometry and the generation of specific mAbs for different epitopes related to phenotypic and functional parameters have facilitated our understanding of NK cell responses. Here, we provide protocols for flow cytometric evaluation of degranulation and cytokine production by human NK cells from peripheral blood at the single-cell level. In addition to offering insight into the regulation of human NK cell responses, these techniques are applicable to the assessment of various clinical conditions, including the diagnosis of immunodeficiency syndromes.
自然杀伤（NK）细胞是淋巴细胞的一个亚群，通过细胞因子分泌和靶细胞裂解作用参与固有免疫。NK细胞功能受多种激活和抑制性受体调节。多色流式细胞术检测仪器的进步以及针对与表型和功能参数相关的不同表位产生的特异性单克隆抗体，有助于我们理解NK细胞反应。在此，我们提供了在单细胞水平上对外周血人NK细胞脱颗粒和细胞因子产生进行流式细胞术评估的方案。除了有助于深入了解人NK细胞反应的调节机制外，这些技术还可用于评估各种临床状况，包括免疫缺陷综合征的诊断。
