采用多重聚合酶链反应-序列特异性引物法进行杀伤细胞免疫球蛋白样受体基因分型。
KIR genotyping by multiplex PCR-SSP.
作者信息
Kulkarni Smita, Martin Maureen P, Carrington Mary
机构信息
Cancer and Inflammation Program, Laboratory of Experimental Immunology, SAIC-Frederick Inc., National Cancer Institute-Frederick, Frederick, MD, USA.
出版信息
Methods Mol Biol. 2010;612:365-75. doi: 10.1007/978-1-60761-362-6_25.
Abstract
Diversity across KIR haplotypes stems from differences in numbers of inhibitory and activating receptors, as well as allelic polymorphism of individual genes. The KIR locus has undergone large expansions and contractions over time and is believed to be coevolving with genes encoding its HLA class I ligands located within the MHC locus. KIR and HLA compound genotypes have been associated with susceptibility to or protection from infectious, autoimmune, reproductive, and malignant disorders. We describe here a simple and reliable multiplex PCR-SSP (sequence-specific priming) method for relatively rapid and inexpensive genotyping of 15 KIR genes using standard agarose gel electrophoresis.
摘要
KIR单倍型的多样性源于抑制性和激活性受体数量的差异，以及单个基因的等位基因多态性。随着时间的推移，KIR基因座经历了大规模的扩张和收缩，并且被认为与位于MHC基因座内编码其HLA I类配体的基因共同进化。KIR和HLA复合基因型与感染性、自身免疫性、生殖性和恶性疾病的易感性或保护性相关。我们在此描述一种简单可靠的多重PCR-SSP（序列特异性引物）方法，用于使用标准琼脂糖凝胶电泳对15个KIR基因进行相对快速且廉价的基因分型。
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本文引用的文献
1
NK receptor interactions with MHC class I molecules in pregnancy.
Semin Immunol. 2008 Dec;20(6):317-20. doi: 10.1016/j.smim.2008.06.002. Epub 2008 Jul 24.
2
The Yin and Yang of HLA and KIR in human disease.
Semin Immunol. 2008 Dec;20(6):343-52. doi: 10.1016/j.smim.2008.06.003. Epub 2008 Jul 16.
3
KIR locus polymorphisms: genotyping and disease association analysis.
Methods Mol Biol. 2008;415:49-64. doi: 10.1007/978-1-59745-570-1_3.
4
KIR and disease: a model system or system of models?
Immunol Rev. 2006 Dec;214:186-201. doi: 10.1111/j.1600-065X.2006.00459.x.
5
The impact of variation at the KIR gene cluster on human disease.
Curr Top Microbiol Immunol. 2006;298:225-57. doi: 10.1007/3-540-27743-9_12.
6
NK cell recognition.
Annu Rev Immunol. 2005;23:225-74. doi: 10.1146/annurev.immunol.23.021704.115526.
7
The silent KIR3DP1 gene (CD158c) is transcribed and might encode a secreted receptor in a minority of humans, in whom the KIR3DP1, KIR2DL4 and KIR3DL1/KIR3DS1 genes are duplicated.
Eur J Immunol. 2005 Jan;35(1):16-24. doi: 10.1002/eji.200425493.
8
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Trends Genet. 2004 Apr;20(4):196-205. doi: 10.1016/j.tig.2004.02.006.
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10
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