Jiang Mingyan, Guo Xia, Yuan Lixing, Gao Ju, Huo Li, Li Qiang
Department of Pediatric Hematology and Oncology, West China Second University Hospital, Sichuan University Chengdu, Sichuan Province, China.
Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education Chengdu, Sichuan Province, China.
Int J Clin Exp Pathol. 2020 Mar 1;13(3):536-542. eCollection 2020.
B-cell acute lymphoblastic leukemia (B-ALL) is the most common malignancy in children. Killer cell immunoglobulin-like receptors (KIRs) are mainly expressed on natural killer (NK) cells and regulate killing of cancer cells. To investigate the possible association of genes with B-ALL in Chinese children, we used polymerase chain reaction with sequence-specific primers (PCR-SSP) to determine the genotypes of 137 B-ALL patients and 288 healthy children of Chinese Han origin. Herein we report no significant difference in the carrying frequency of individual genes and haplotypes between patients and controls; however, individuals carrying genotypes were more frequent in the B-ALL group compared with healthy controls (11.7% vs. 5.9%, =0.038). In addition, the centromeric gene cluster, , was significantly increased in the B-ALL group compared with healthy controls (13.9% vs. 7.3%, =0.030). These data suggest that the genotype and centromeric gene cluster (KIR2DS2-2DL2-2DS3-2DL5) might predispose to susceptibility to B-ALL in Chinese children.
B细胞急性淋巴细胞白血病（B-ALL）是儿童中最常见的恶性肿瘤。杀伤细胞免疫球蛋白样受体（KIRs）主要表达于自然杀伤（NK）细胞上，并调节对癌细胞的杀伤作用。为了研究基因与中国儿童B-ALL之间可能存在的关联，我们采用序列特异性引物聚合酶链反应（PCR-SSP）来确定137例B-ALL患者以及288例中国汉族健康儿童的基因型。在此我们报告，患者与对照组之间单个基因和单倍型的携带频率无显著差异；然而，与健康对照组相比，携带特定基因型的个体在B-ALL组中更为常见（11.7%对5.9%，P = 0.038）。此外，与健康对照组相比，B-ALL组中着丝粒KIR基因簇（KIR2DS2-2DL2-2DS3-2DL5）显著增加（13.9%对7.3%，P = 0.030）。这些数据表明，特定基因型和着丝粒KIR基因簇（KIR2DS2-2DL2-2DS3-2DL5）可能使中国儿童易患B-ALL。
