2053 个人血样血清磷脂中 ω-3 水平与关键脂肪酸比值的相关性。
Correlation of omega-3 levels in serum phospholipid from 2053 human blood samples with key fatty acid ratios.
机构信息
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
出版信息
Nutr J. 2009 Dec 24;8:58. doi: 10.1186/1475-2891-8-58.
BACKGROUND
This research was conducted to explore the relationships between the levels of omega-3 fatty acids in serum phospholipid and key fatty acid ratios including potential cut-offs for risk factor assessment with respect to coronary heart disease and fatal ischemic heart disease.
METHODS
Blood samples (n = 2053) were obtained from free-living subjects in North America and processed for determining the levels of total fatty acids in serum phospholipid as omega-3 fatty acids including EPA (eicosapentaenoic acid, 20:5 n-3) and DHA (docosahexaenoic acid, 22:6 n-3) by combined thin-layer and gas-liquid chromatographic analyses. The omega-3 levels were correlated with selected omega-6: omega-3 ratios including AA (arachidonic acid, 20:4n-6): EPA and AA:(EPA+DHA). Based on previously-published levels of omega-3 fatty acids considered to be in a 'lower risk' category for heart disease and related fatality, 'lower risk' categories for selected fatty acid ratios were estimated.
RESULTS
Strong inverse correlations between the summed total of omega-3 fatty acids in serum phospholipid and all four ratios (omega-6:omega-3 (n-6:n-3), AA:EPA, AA:DHA, and AA:(EPA+DHA)) were found with the most potent correlation being with the omega-6:omega-3 ratio (R(2) = 0.96). The strongest inverse relation for the EPA+DHA levels in serum phospholipid was found with the omega-6: omega-3 ratio (R(2) = 0.94) followed closely by the AA:(EPA+DHA) ratio at R(2) = 0.88. It was estimated that 95% of the subjects would be in the 'lower risk' category for coronary heart disease (based on total omega-3 >or= 7.2%) with omega-6:omega-3 ratios <4.5 and AA:(EPA+DHA) ratios <1.4. The corresponding ratio cut-offs for a 'lower risk' category for fatal ischemic heart disease (EPA+DHA >or= 4.6%) were estimated at < 5.8 and < 2.1, respectively.
CONCLUSIONS
Strong inverse correlations between the levels of omega-3 fatty acids in serum (or plasma) phospholipid and omega-6: omega-3 ratios are apparent based on this large database of 2053 samples. Certain fatty acid ratios may aid in cardiovascular disease-related risk assessment if/when complete profiles are not available.
背景
本研究旨在探讨血清磷脂中ω-3 脂肪酸水平与关键脂肪酸比值之间的关系，包括冠心病和致命性缺血性心脏病风险评估的潜在临界点。
方法
从北美自由生活的受试者中采集血样（n=2053），通过组合薄层层析和气相色谱分析，测定血清磷脂中总脂肪酸水平，包括 EPA（二十碳五烯酸，20:5n-3）和 DHA（二十二碳六烯酸，22:6n-3）作为 ω-3 脂肪酸。将 ω-3 水平与选定的 ω-6：ω-3 比值（包括 AA（花生四烯酸，20:4n-6）：EPA 和 AA：（EPA+DHA））进行相关性分析。基于先前发表的被认为处于心脏病和相关死亡率“低风险”类别的 ω-3 脂肪酸水平，估计了选定脂肪酸比值的“低风险”类别。
结果
血清磷脂中 ω-3 脂肪酸总量与所有四个比值（ω-6：ω-3（n-6：n-3）、AA：EPA、AA：DHA 和 AA：（EPA+DHA））之间存在强烈的负相关，最强烈的相关性与 ω-6：ω-3 比值（R²=0.96）。血清磷脂中 EPA+DHA 水平与 ω-6：ω-3 比值（R²=0.94）呈最强负相关，紧随其后的是 AA：（EPA+DHA）比值，R²为 0.88。据估计，95%的受试者将处于冠心病（基于总 ω-3 >或=7.2%）的“低风险”类别，ω-6：ω-3 比值<4.5，AA：（EPA+DHA）比值<1.4。致命性缺血性心脏病（EPA+DHA >或=4.6%）的“低风险”类别的相应比值截止值估计分别为<5.8 和<2.1。
结论
基于这个包含 2053 个样本的大型数据库，明显存在血清（或血浆）磷脂中 ω-3 脂肪酸水平与 ω-6：ω-3 比值之间的强烈负相关。如果/当无法获得完整图谱时，某些脂肪酸比值可能有助于心血管疾病相关风险评估。
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