Tang Wei, Peng Kang, Zhu Chuan-wu, Lei Xiao-lin, Liu Wei
Department of Chinese Medicine and Formula, College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Dec;29(12):2421-4.
To evaluate the effect of Wulongdan, a tradition Chinese medicinal preparation, on the learning and memory abilities of rats with chronic cerebral ischemia and explore the mechanisms.
Male rats with chronic cerebral ischemia induced by permanent ligation of the bilateral carotid arteries were randomized into sham-operated group, chronic cerebral ischemia (model) group, and high-, middle-, and low-dose Wulongdan groups and Yinxingye group. The corresponding treatments were administered in the rats 24 h after the operation once daily for 8 consecutive weeks. Morris water maze and step-through tests were performed after 7 weeks of drug administration. The brain tissues were then taken to observe the morphological changes in the hippocampal neurons with Nissl staining by transmission electron microscopy.
Compared with the model group, the latency of finding the platform in Morris water maze test was significantly shortened (P<0.05 or 0.01), and that in step-through test significantly prolonged (P<0.05 or 0.01) in high-, middle-, and low-dose Wulongdan groups and Yinxingye group. In the model group, Nissl staining of the hippocampal CA1 region visualized obvious pathological changes in the neurons, showing a significant difference from the sham-operated and high-dose Wulongdan groups.
Wulongdan can enhance the learning and memory abilities of rats with chronic cerebral ischemia possibly through a mechanism in relation to neuronal protection in the hippocampus CA1 region.
评价中药制剂乌龙丹对慢性脑缺血大鼠学习记忆能力的影响并探讨其作用机制。
采用永久性结扎双侧颈总动脉法制备慢性脑缺血雄性大鼠模型，将其随机分为假手术组、慢性脑缺血（模型）组、乌龙丹高、中、低剂量组及银杏叶组。术后24 h开始给药，每日1次，连续8周。给药7周后进行Morris水迷宫试验和跳台试验。然后取脑组织，通过透射电子显微镜尼氏染色观察海马神经元的形态变化。
与模型组相比，乌龙丹高、中、低剂量组及银杏叶组在Morris水迷宫试验中找到平台的潜伏期显著缩短（P<0.05或0.01），在跳台试验中的潜伏期显著延长（P<0.05或0.01）。模型组海马CA1区神经元尼氏染色显示明显病理改变，与假手术组和乌龙丹高剂量组有显著差异。
乌龙丹可能通过对海马CA1区神经元保护机制增强慢性脑缺血大鼠的学习记忆能力。
