Tang Wei, Peng Kang, Yang Zhen-hui, Diao Jian-xin, Hu Chuan-wu, Lei Xiao-lin
Medical College of Jishou University, Jishou 416000, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Oct;30(10):2333-5, 2339.
To evaluate the effect of Wulongdan on the learning and memory abilities of rats with chronic cerebral ischemia and explore the mechanisms.
Male SD Rat models of chronic cerebral ischemia were established by permanent ligation of the bilateral carotid arteries. Three weeks after the operation, the rats were randomly divided into sham-operated group, chronic cerebral ischemia group (model group), high-dose drug group, low-dose drug group and Yinxingye group and received the corresponding treatments on a daily basis for 5 consecutive weeks. Morris water maze was used to assess the learning and memory abilities of the rats, and Western blotting was carried out for detecting the expressions of NR1 and NR2B in the hippocampus and cerebral cortex.
Compared with the model group, the rats in high-dose drug, low-dose drug and Yinxingye groups showed significantly shorter time of finding platform in Morris water maze test (P<0.05 or 0.01). The rats in the model group showed significantly lowered expressions of NR1 and NR2B of the cortex and hippocampus than those in the sham-operated group (P<0.01). In comparison with the model group, the high-dose Wulongdan group and Yinxingye group both showed significantly increase expressions of NR1 and NR2B (P<0.01), but their expression levels still remained significantly lower than those in the sham-operated group (P<0.01).
Wulongdan can enhance the learning and memory abilities of rats with chronic cerebral ischemia, the mechanisms of which may involve increased expressions of NR1 and NR2B in the hippocampus and cortex.
评价乌龙丹对慢性脑缺血大鼠学习记忆能力的影响并探讨其机制。
采用双侧颈总动脉永久性结扎法建立雄性SD大鼠慢性脑缺血模型。术后3周，将大鼠随机分为假手术组、慢性脑缺血组（模型组）、高剂量药物组、低剂量药物组和银杏叶组，连续5周每日给予相应处理。采用Morris水迷宫评估大鼠的学习记忆能力，并用蛋白质免疫印迹法检测海马和大脑皮质中NR1和NR2B的表达。
与模型组相比，高剂量药物组、低剂量药物组和银杏叶组大鼠在Morris水迷宫试验中找到平台的时间明显缩短（P<0.05或0.01）。模型组大鼠皮质和海马中NR1和NR2B的表达明显低于假手术组（P<0.01）。与模型组相比，高剂量乌龙丹组和银杏叶组NR1和NR2B的表达均明显增加（P<0.01），但其表达水平仍明显低于假手术组（P<0.01）。
乌龙丹可提高慢性脑缺血大鼠的学习记忆能力，其机制可能与海马和皮质中NR1和NR2B表达增加有关。
