Pharmaceutical Science Division, School of Biomedical and Health Sciences, King's College, London, SE1 9NH, United Kingdom.
Pediatr Res. 2010 Apr;67(4):412-8. doi: 10.1203/PDR.0b013e3181d026c3.
The role of infection in bronchopulmonary dysplasia (BPD) is unknown. We present an observational study of 55 premature infants born weighing less than 1.3 kg within two level III neonatal intensive care units. Endotracheal aspirates (ETA) and nasogastric aspirates (NGA) were studied with denaturing gradient gel electrophoresis (DGGE) profiling to elucidate the total bacterial community, and species-specific PCR was used to detect the presence of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. DGGE identified bacterial species in 59% of NGA and ETA samples combined. A diverse range of species were identified including several implicated in preterm labor. Species-specific PCR identified M. hominis in 25% of NGA and 11% of ETA samples. Among the 48 infants surviving up to 36 wk-postconceptual age, ordinal logistic regression showed the odds ratio for BPD or death where Ureaplasma was present/absent as 4.80 (95% CI 1.15-20.13). After adjusting for number of days ventilated, this was reduced to 2.04 (0.41-10.25). These data demonstrate how the combined use of DGGE and species-specific PCR identifies a high exposure in utero and around the time of birth to bacteria that might be causally related to preterm delivery and subsequent lung injury.
感染在支气管肺发育不良（BPD）中的作用尚不清楚。我们进行了一项观察性研究，共纳入了 55 名出生体重不足 1.3 公斤的早产儿，他们均来自于 2 个 3 级新生儿重症监护病房。我们使用变性梯度凝胶电泳（DGGE）分析了气管内吸出物（ETA）和鼻胃吸出物（NGA），以阐明总细菌群落，并用种特异性 PCR 检测人型支原体、解脲脲原体和微小脲原体的存在情况。DGGE 联合检测了 59%的 NGA 和 ETA 样本中的细菌种类。鉴定出了多种细菌，其中包括一些与早产有关的细菌。种特异性 PCR 在 25%的 NGA 和 11%的 ETA 样本中检测到了人型支原体。在 48 名存活至 36 周龄的婴儿中，有序逻辑回归显示，存在/不存在脲原体时，BPD 或死亡的优势比为 4.80（95%CI 1.15-20.13）。在调整了通气天数后，该比值降低至 2.04（0.41-10.25）。这些数据表明，DGGE 和种特异性 PCR 的联合使用可以鉴定出在宫内和出生前后高暴露于可能与早产和随后的肺损伤有关的细菌。
