Department of Animal Pathology, Hygiene and Veterinary Public Health, Via Celoria 10, Milano, Italy.
Vet Parasitol. 2010 Mar 25;168(3-4):293-8. doi: 10.1016/j.vetpar.2009.12.001. Epub 2009 Dec 11.
The acute phase proteins (APP) are a group of serum proteins that change their concentration in animals following external or internal challenges, such as infection, inflammation or stress. The concentrations of four APPs, including serum amyloid A (SAA), haptoglobin (Hp), alpha(1)-acid glycoprotein (AGP) and ceruloplasmin (Cp) were determined in serum collected from healthy Alpine ibexes (Capra ibex) and ibexes with Sarcoptes scabiei mange. Primary structures of all four APPs were determined by cDNA sequencing. The concentrations of all four APPs were higher in serum of animals with clinical signs of sarcoptic mange when compared to healthy animals. Two of the APPs, including SAA and AGP, acted as major APPs, since their serum concentrations were increased more than 10-folds when compared to healthy animals (P<0.001). The other two APPs, including Hp and Cp, acted as minor acute phase proteins, as their concentrations were increased from two to five folds (P<0.001). These findings provide a remarkable potential as diagnostic markers for the early detection of sarcoptic mange in free ranging animals.
急性期蛋白（APP）是一组血清蛋白，在动物受到感染、炎症或应激等外部或内部挑战后，其浓度会发生变化。本研究测定了来自健康阿尔卑斯野山羊（Capra ibex）和患有疥螨病的野山羊血清中 4 种 APP（血清淀粉样蛋白 A（SAA）、触珠蛋白（Hp）、α(1)-酸性糖蛋白（AGP）和铜蓝蛋白（Cp））的浓度。通过 cDNA 测序确定了这 4 种 APP 的一级结构。与健康动物相比，患有临床症状的疥螨病动物的血清中这 4 种 APP 的浓度均升高。其中 2 种 APP（SAA 和 AGP）为主要的 APP，其血清浓度比健康动物高 10 倍以上（P<0.001）。另外 2 种 APP（Hp 和 Cp）为次要的急性期蛋白，其浓度增加了 2 到 5 倍（P<0.001）。这些发现为在自由放养动物中早期检测疥螨病提供了非常有潜力的诊断标志物。
