The Schubot Exotic Bird Health Center, Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77840, USA.
Vet Microbiol. 2010 Jul 14;143(2-4):196-201. doi: 10.1016/j.vetmic.2009.11.041. Epub 2009 Dec 4.
Avian Borna virus (ABV) has recently been shown to be the causal agent of proventricular dilatation disease (PDD) a lethal neurologic disease of captive psittacines and other birds. An immunoblot assay was used to detect the presence of antibodies against avian Borna virus in the serum of affected birds. A lysate from ABV-infected duck embryo fibroblasts served as a source of antigen. The assay was used to test for the presence of antibodies to ABV in 117 birds. Thirty of these birds had biopsy or necropsy-confirmed proventricular dilatation disease (PDD), while the remaining 87 birds were apparently healthy or were suffering from diseases other than PDD. Sera from 27 of the 30 PDD cases (90%) contained antibodies to ABV. Seventy-three (84%) of the apparently "healthy" birds were seronegative. Additionally, sera from seven macaws and one parrot trapped in the Peruvian Amazon were seronegative. Positive sera recognized the bornaviral nucleoprotein (N-protein). While the presence of antibodies to ABV largely corresponded with the development of clinical PDD, 14 apparently healthy normal birds possessed detectable antibodies to ABV. The existence of a carrier state was confirmed when 13 of 15 apparently healthy cockatiels were shown by PCR to have detectable ABV RNA in their feces. Western blot assays may be of significant assistance in diagnosing proventricular dilatation disease. Many apparently healthy birds may however be seronegative while, at the same time, shedding ABV in their feces.
禽博纳病毒（ABV）最近被证实是前胃扩张病（PDD）的病原体，这是一种致命的鹦鹉和其他鸟类的神经疾病。免疫印迹分析用于检测受感染鸟类血清中针对禽博纳病毒的抗体存在情况。ABV 感染的鸭胚成纤维细胞裂解物用作抗原来源。该检测方法用于检测 117 只鸟类中是否存在针对 ABV 的抗体。这些鸟类中有 30 只具有活检或尸检确认的前胃扩张病（PDD），而其余 87 只鸟类显然健康或患有除 PDD 以外的其他疾病。30 个 PDD 病例中的 27 个（90%）血清中含有针对 ABV 的抗体。73 只（84%）显然“健康”的鸟类血清呈阴性。此外，在秘鲁亚马逊捕获的 7 只金刚鹦鹉和 1 只长尾鹦鹉的血清均呈阴性。阳性血清识别出博纳病毒的核蛋白（N 蛋白）。虽然存在针对 ABV 的抗体与临床 PDD 的发展有很大的相关性，但 14 只显然健康的正常鸟类也具有可检测到的针对 ABV 的抗体。当通过 PCR 显示 15 只显然健康的鸡尾鹦鹉中有 13 只粪便中可检测到 ABV RNA 时，确认了携带状态的存在。Western blot 分析可能对诊断前胃扩张病有很大帮助。然而，许多显然健康的鸟类可能呈血清阴性，但同时在粪便中排出 ABV。
