美国东北部疣鼻天鹅中广泛存在禽源博尔纳病毒感染。
Widespread avian bornavirus infection in mute swans in the Northeast United States.
作者信息
Guo Jianhua, Covaleda Lina, Heatley J Jill, Baroch John A, Tizard Ian, Payne Susan L
机构信息
Texas A&M University, College Station, TX, USA,
USDA/APHIS Wildlife Services, Fort Collins, CO, USA.
出版信息
Vet Med (Auckl). 2012 Jul 19;3:49-52. doi: 10.2147/VMRR.S33353. eCollection 2012.
Abstract
Avian bornavirus (ABV) matrix (M) genes were detected by RT-PCR on brain tissue obtained from 192 mute swans harvested from several Northeastern states. A RT-PCR product was detected in 45 samples. Sequencing of the PCR products confirmed the presence of ABV belonging to the 'goose' genotype. The prevalence of positive samples ranged from 28% in Michigan to 0% in northern New York State. Two Rhode Island isolates were cultured. Their M, N, and X-P gene sequences closely matched recently published sequences from Canada geese.
摘要
通过逆转录聚合酶链反应（RT-PCR）对从美国东北部几个州采集的192只疣鼻天鹅的脑组织进行检测，以检测禽源博尔纳病毒（ABV）的基质（M）基因。在45个样本中检测到RT-PCR产物。对PCR产物进行测序，证实存在属于“鹅”基因型的ABV。阳性样本的患病率从密歇根州的28%到纽约州北部的0%不等。培养了两个罗德岛分离株。它们的M、N和X-P基因序列与最近发表的加拿大鹅的序列密切匹配。
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