连翘提取物对百草枯诱导的大鼠氧化应激的保护作用。

Protective effects of Forsythia suspensa extract against oxidative stress induced by diquat in rats.

机构信息

State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2, West Road, Yuanmingyuan, Beijing 100193, China.

出版信息

Food Chem Toxicol. 2010 Feb;48(2):764-70. doi: 10.1016/j.fct.2009.12.018. Epub 2009 Dec 28.

DOI:10.1016/j.fct.2009.12.018

PMID:20036301

Abstract

Forsythia suspensa extract has been proved as a potential antioxidant in the recent years. The present study was undertaken to obtain the optimal antioxidant fraction in vitro and examine its antioxidative potential against diquat-induced oxidative stress in male Sprague Dawley rats in vivo. In vitro, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging experiment indicated that the CH2Cl2 fraction of F. suspensa (FSC) exerted the strongest scavenging activities; forsythoside A, forythialan A and phillygenin from it might be the major antioxidant constituents. In vivo, pretreatment of rats with different doses of FSC (25, 50 and 100 mg/kg bw) and vitamin C (100 mg/kg bw, positive control) for 15 days significantly lowered the tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) in plasma compared to the negative control group. Also, FSC significantly increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and the levels of glutathione (GSH) in plasma, liver and kidney whereas it decreased the levels of malondialdehyde (MDA) in plasma and kidney. Moreover, the protective effect of FSC (100 mg/kg bw) was better than vitamin C. These results revealed that FSC exerted a protective effect against diquat-induced oxidative stress and is worthy of becoming a potential dietary antioxidant.

摘要

连翘提取物近年来已被证明具有潜在的抗氧化作用。本研究旨在获得体外最佳抗氧化部分，并研究其对体内雄性 Sprague Dawley 大鼠二氯喹啉诱导氧化应激的抗氧化潜力。体外，1,1-二苯基-2-苦基肼（DPPH）自由基清除实验表明，连翘（FSC）的 CH2Cl2 部分表现出最强的清除活性；其中的连翘苷 A、连翘苷 A 和连翘苷可能是主要的抗氧化成分。体内，用不同剂量的 FSC（25、50 和 100mg/kg bw）和维生素 C（100mg/kg bw，阳性对照）预处理大鼠 15 天，与阴性对照组相比，显著降低了血浆中的肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）。此外，FSC 还显著提高了血浆、肝脏和肾脏中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性以及谷胱甘肽（GSH）的水平，同时降低了血浆和肾脏中丙二醛（MDA）的水平。此外，FSC（100mg/kg bw）的保护作用优于维生素 C。这些结果表明，FSC 对二氯喹啉诱导的氧化应激具有保护作用，有望成为一种潜在的膳食抗氧化剂。