Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China.
Cell Stem Cell. 2010 Jan 8;6(1):71-9. doi: 10.1016/j.stem.2009.12.001. Epub 2009 Dec 31.
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.
体细胞可以通过特定的因子重编程为诱导多能干细胞(iPSCs)。然而，重编程过程的低效率和缓慢的动力学阻碍了该技术的发展。在这里，我们报告一种天然化合物，维生素 C（Vc），可以增强来自小鼠和人类体细胞的 iPSC 的生成。Vc 的作用至少部分是通过减轻细胞衰老来实现的，细胞衰老最近被确定为重编程的一个障碍。此外，Vc 还加速了基因表达的变化，并促进了前 iPSC 集落向完全重编程状态的转变。因此，我们的研究结果突出了一种提高 iPSC 生成速度和效率的简单方法，并为重编程过程的机制基础提供了更多的见解。
