Zhu Fei, Nie Guangjun
Wisdom Lake Academy of Pharmacy, Xi'an Jiaotong-Liverpool University, Suzhou, 215123, China.
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center of Excellence in Nanoscience National Center for Nanoscience and Technology, Beijing, 100190, China.
Cell Regen. 2025 Mar 27;14(1):12. doi: 10.1186/s13619-025-00229-x.
Cell reprogramming represents a powerful approach to achieve the conversion cells of one type into cells of another type of interest, which has substantially changed the landscape in the field of developmental biology, regenerative medicine, disease modeling, drug discovery and cancer immunotherapy. Cell reprogramming is a complex and ordered process that involves the coordination of transcriptional, epigenetic, translational and metabolic changes. Over the past two decades, a range of questions regarding the facilitators/barriers, the trajectories, and the mechanisms of cell reprogramming have been extensively investigated. This review summarizes the recent advances in cell reprogramming mediated by transcription factors or chemical molecules, followed by elaborating on the important roles of biophysical cues in cell reprogramming. Additionally, this review will detail our current understanding of the mechanisms that govern cell reprogramming, including the involvement of the recently discovered biomolecular condensates. Finally, the review discusses the broad applications and future directions of cell reprogramming in developmental biology, disease modeling, drug development, regenerative/rejuvenation therapy, and cancer immunotherapy.
细胞重编程是一种将一种类型的细胞转化为另一种目标类型细胞的有效方法，它极大地改变了发育生物学、再生医学、疾病建模、药物发现和癌症免疫治疗领域的格局。细胞重编程是一个复杂且有序的过程，涉及转录、表观遗传、翻译和代谢变化的协调。在过去二十年中，一系列关于细胞重编程的促进因素/障碍、轨迹和机制的问题得到了广泛研究。本综述总结了由转录因子或化学分子介导的细胞重编程的最新进展，随后阐述了生物物理线索在细胞重编程中的重要作用。此外，本综述将详细介绍我们目前对控制细胞重编程机制的理解，包括最近发现的生物分子凝聚物的参与。最后，综述讨论了细胞重编程在发育生物学、疾病建模、药物开发、再生/恢复活力疗法和癌症免疫治疗中的广泛应用和未来方向。
