Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
Acta Biomater. 2010 Jul;6(7):2721-31. doi: 10.1016/j.actbio.2009.12.043. Epub 2009 Dec 29.
We report here the in vitro cytotoxicity of mesoporous silicon (PSi) microparticles on the Caco-2 cells as a function of particle size fractions (1.2-75 microm), particle concentration (0.2-4 mg ml(-1)) and incubation times (3, 11 and 24 h). The particle size (smaller PSi particles showed higher cytotoxicity) and the surface chemistry treatment of the PSi microparticles were considered to be the key factors regarding the toxicity aspects. These effects were significant after the 11 and 24 h exposure times, and were explained by cell-particle interactions involving mitochondrial disruption resulting from ATP depletion and reactive oxygen species production induced by the PSi surface. These events further induced an increase in cell apoptosis and consequent cell damage and cell death in a dose-dependent manner and as a function of the PSi particle size. These effects were, however, less pronounced with thermally oxidized PSi particles. Under the experimental conditions tested and at particle sizes >25 microm, the non-toxic threshold concentration for thermally hydrocarbonized and carbonized PSi particles was <2 mg ml(-1), and for thermally oxidized PSi microparticles was <4 mg ml(-1).
我们在此报告介孔硅（PSi）微球的体外细胞毒性，其作为粒径分数（1.2-75μm）、颗粒浓度（0.2-4mg/ml）和孵育时间（3、11 和 24 小时）的函数。颗粒尺寸（较小的 PSi 颗粒显示出更高的细胞毒性）和 PSi 微球的表面化学处理被认为是毒性方面的关键因素。这些影响在 11 和 24 小时暴露时间后变得显著，并可通过涉及由于 ATP 耗竭而导致的线粒体破坏以及由 PSi 表面诱导的活性氧物种产生的细胞-颗粒相互作用来解释。这些事件进一步以剂量依赖的方式和 PSi 颗粒尺寸的函数诱导细胞凋亡和随后的细胞损伤和细胞死亡增加。然而，在用热氧化 PSi 颗粒的情况下，这些效果不太明显。在测试的实验条件下和在颗粒尺寸>25μm 的情况下，热烃化和碳化 PSi 颗粒的无毒阈值浓度<2mg/ml，而对于热氧化 PSi 微球则<4mg/ml。
