Belfort-Mattos Patrícia Napoli, Focchi Gustavo Rubino de Azevedo, Ribalta Julisa Chamorro Lascasas, Megale De Lima Tatiana, Nogueira Carvalho Carmen Regina, Kesselring Tso Fernanda, De Góis Speck Neila Maria
Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, UNIFESP/EPM, 380 Borges Lagoa Street, 04038-000 São Paulo, SP, Brazil.
Department of Pathology, Federal University of São Paulo, UNIFESP/EPM, 740 Botucatu Street, 04023-062 São Paulo, SP, Brazil.
Dis Markers. 2016;2016:8293196. doi: 10.1155/2016/8293196. Epub 2016 May 23.
VEGF and podoplanin (PDPN) have been identified as angiogenesis and/or lymphangiogenesis regulators and might be essential to restrict tumor growth, progression, and metastasis. In the present study, we evaluate the association between the expression of these markers and CIN grade. Immunohistochemistry was performed in 234 uterine cervical samples using conventional histologic sections or TMA with the monoclonal antibodies to VEGF (C-1 clone) and podoplanin (D2-40 clone). Positive-staining rates of VEGF in 191 CIN specimens were significantly associated with histological grade (P < 0.001). Negative and/or focal immunostaining for PDPN were more frequent in CIN 3 (P = 0.016). We found that patients with CIN 3 more frequently had strong and more diffuse staining for VEGF and diminished staining for PDPN (P = 0.018). Strong and more diffuse VEGF immunoexpressions in CIN 2 and CIN 3 were detected when compared to CIN 1. Negative and/or focal PDPN immunoexpression appear to be more frequent in CIN 3. Moderate to strong VEGF expression may be a tendency among patients with high-grade lesions and diminished PDPN expression.
血管内皮生长因子（VEGF）和血小板源性生长因子（PDPN）已被确定为血管生成和/或淋巴管生成的调节因子，可能对限制肿瘤生长、进展和转移至关重要。在本研究中，我们评估了这些标志物的表达与宫颈上皮内瘤变（CIN）分级之间的关联。使用针对VEGF（C-1克隆）和血小板源性生长因子（D2-40克隆）的单克隆抗体，对234例子宫颈样本进行常规组织切片或组织微阵列的免疫组织化学检测。191例CIN标本中VEGF的阳性染色率与组织学分级显著相关（P < 0.001）。CIN 3中血小板源性生长因子的阴性和/或局灶性免疫染色更为常见（P = 0.016）。我们发现，CIN 3患者VEGF染色更强且更弥漫，血小板源性生长因子染色减弱（P = 0.018）。与CIN 1相比，CIN 2和CIN 3中VEGF免疫表达更强且更弥漫。CIN 3中血小板源性生长因子阴性和/或局灶性免疫表达似乎更为常见。高级别病变患者可能倾向于有中度至强的VEGF表达，而血小板源性生长因子表达减弱。
