Laboratory of Biochemistry and Molecular Biology, Carthage University, Faculty of Sciences of Bizerte, Zarzouna, Bizerte, Tunisia.
Environmental Research Institute and School of Biochemistry and Cell Biology, University College Cork, Western Gateway Building, Western Road, Cork, Ireland.
PLoS One. 2019 Jan 4;14(1):e0209843. doi: 10.1371/journal.pone.0209843. eCollection 2019.
Exposure to static magnetic fields (SMF) can cause changes in microorganism metabolism altering key subcellular functions. The purpose of this study was to investigate whether an applied SMF could induce biological effects on growth of Saccharomyces cerevisiae, and then to probe biochemical and bio-molecular responses. We found a decrease in growth and viability under SMF (250mT) after 6h with a significant decrease in colony forming units followed by an increase between 6 h and 9 h. Moreover, measurements of antioxidant enzyme activities (catalase, superoxide dismutase, glutathione peroxidase) demonstrated a particular profile suggesting oxidative stress. For instance, SOD and catalase activities increased in magnetized cultures after 9 h compared with unexposed samples. However, SMF exposure caused a decrease in glutathione peroxidase activity. Finally, SMF caused an increase in MDA levels as well as the content of protein carbonyl groups after 6 and 9 h of exposure.
暴露于静磁场（SMF）会引起微生物代谢变化，从而改变关键的细胞内功能。本研究的目的是研究施加的 SMF 是否会对酿酒酵母的生长产生生物效应，然后探究其生化和生物分子反应。我们发现，在 SMF（250mT）下孵育 6 小时后，生长和活力下降，随后菌落形成单位显著减少，在 6 小时至 9 小时之间增加。此外，抗氧化酶活性（过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶）的测量表明存在特定的氧化应激模式。例如，与未暴露的样品相比，磁化培养物中 SOD 和过氧化氢酶的活性在 9 小时后增加。然而，SMF 暴露会导致谷胱甘肽过氧化物酶活性降低。最后，SMF 导致 MDA 水平以及 6 小时和 9 小时暴露后蛋白质羰基含量增加。
