Feed Safety Reference Laboratory of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
J Agric Food Chem. 2010 Jan 27;58(2):943-8. doi: 10.1021/jf9026316.
Many countries have introduced maximum residue limits for melamine in foods since its adulteration in infant formula in China. However, more animal feeding studies are needed to understand the fate of melamine-contaminated animal feed. In this study, the melamine contents in tissues and serum were tested using LC-MS/MS for up to 60 days for lambs fed with diets containing 2-100 mg of melamine per kg of diet. A higher dose of melamine in the diet resulted in a higher concentration in tissues and serum, with the maximum melamine content in the kidney. When cyanuric acid was coadministered at an equal concentration to that of melamine, the deposition of melamine in lambs was similar to the treatment with melamine only and was much higher than the deposition of cyanuric acid. When melamine was withdrawn from the diet, melamine concentrations decreased below 20 microg/kg in all tissues after 4 days. The present study may provide available information for future work about the risk assessment of melamine to human health.
许多国家在中国婴儿配方奶粉掺加三聚氰胺后，都对食品中的三聚氰胺残留量制定了最高限量标准。然而，为了了解受三聚氰胺污染的动物饲料的命运，还需要更多的动物饲养研究。在这项研究中，用 LC-MS/MS 法检测了摄入含 2-100mg/kg 三聚氰胺日粮的羔羊组织和血清中的三聚氰胺含量，检测时间长达 60 天。日粮中三聚氰胺的剂量越高，组织和血清中的浓度就越高，三聚氰胺在肾脏中的含量最高。当三聚氰胺与与等浓度的三聚氰酸同时给予时，三聚氰胺在羔羊体内的沉积与单独给予三聚氰胺的处理相似，且明显高于三聚氰酸的沉积。当从日粮中撤出三聚氰胺后，4 天后所有组织中的三聚氰胺浓度均低于 20μg/kg。本研究可为今后三聚氰胺对人类健康风险评估的相关工作提供可用信息。
