Faculty of Biosciences and Aquaculture, Bodø University College, NO-8049 Bodø, Norway.
J Exp Biol. 2010 Jan 15;213(2):200-9. doi: 10.1242/jeb.033126.
In Senegalese sole (Solea senegalensis Kaup), growth is negatively correlated to dietary lipid levels. To understand the molecular basis of this effect a molecular toolbox of 12 genes, including fgf6, fst, mstn1, myf5, mrf4, myod1, myod2, myog, myHC, mylc2, igf1r and insr, was developed. The expression profiles of these genes were investigated in white muscle and liver of fish fed with three dietary lipid levels (4%, 12% and 20%). The expression of igf-I and igf-II was also examined. MRFs and myosins were only expressed in the muscle and, except for myf5, the general trend was a decrease in expression with an increase in dietary lipids. Fgf6 was identified for the first time in liver and its expression augmented in hepatic tissues with increasing dietary lipid levels. A similar tendency was observed for mstn1 and igf-I. The opposite was observed for igf1r expression in muscle and liver. Myog, mrf4, mylc2 and igf1r were highly correlated with growth and nutrient utilisation indices. In addition to its practical implications, this work provides a valuable contribution towards our understanding of the genetic networks controlling growth in teleosts.
在塞内加尔比目鱼（Solea senegalensis Kaup）中，生长与饮食脂质水平呈负相关。为了了解这种影响的分子基础，我们开发了一个包含 12 个基因的分子工具包，包括 fgf6、fst、mstn1、myf5、mrf4、myod1、myod2、myog、myHC、mylc2、igf1r 和 insr。研究了这些基因在摄食三种不同脂肪水平（4%、12%和 20%）的鱼类白肌和肝脏中的表达谱。还检查了 igf-I 和 igf-II 的表达情况。MRFs 和肌球蛋白仅在肌肉中表达，除了 myf5 之外，随着饮食脂质的增加，表达趋势呈下降趋势。首次在肝脏中鉴定出 fgf6，其表达随着饮食脂质水平的增加而增加。mstn1 和 igf-I 也表现出类似的趋势。肌肉和肝脏中 igf1r 的表达则相反。Myog、mrf4、mylc2 和 igf1r 与生长和营养利用指数高度相关。除了其实际意义外，这项工作还为我们理解控制硬骨鱼类生长的遗传网络提供了有价值的贡献。
