Graduate Entry Medical School and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland.
Electrophoresis. 2010 Jan;31(1):55-64. doi: 10.1002/elps.200900467.
The theme of this review is to highlight the importance of microscale electrophoretic-based separation systems in microdialysis (microD). The ability of CE and MCE to yield very rapid and highly efficient separations using just nanolitre volumes of microdialysate samples will also be discussed. Recent advances in this area will be highlighted, by illustration of some exciting new applications while the need for further innovation will be covered. The first section briefly introduces the concept of microD sampling coupled with electrophoresis-based separation and the inherent advantages of this approach. The following section highlights some specific applications of CE separations in the detection of important biomarkers such as low-molecular-weight neurotransmitters, amino acids, and other molecules that are frequently encountered in microD. Various detection modes in CE are outlined and some of the advantages and drawbacks thereof are discussed. The last section introduces the concepts of micro-total analysis systems and the coupling of MCE and microD. Some of the latest innovations will be illustrated. The concluding section reflects on the future of this important chemical alliance between microD and CE/MCE.
本文综述的主题是强调基于微尺度电泳的分离系统在微透析（microD）中的重要性。还将讨论 CE 和 MCE 仅使用纳升级微透析样品体积即可实现非常快速和高效分离的能力。通过说明一些令人兴奋的新应用，突出该领域的最新进展，同时涵盖进一步创新的必要性。第一部分简要介绍了与电泳分离相结合的微透析采样的概念以及这种方法的固有优势。接下来的部分重点介绍了 CE 分离在检测重要生物标志物（如低分子量神经递质、氨基酸和其他在 microD 中经常遇到的分子）中的一些具体应用。概述了 CE 中的各种检测模式，并讨论了它们的一些优点和缺点。最后一部分介绍了微全分析系统的概念以及 MCE 和 microD 的耦合。将举例说明一些最新的创新。最后一部分反映了 microD 和 CE/MCE 之间这一重要化学联盟的未来。
