Department of Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, KS 66047, USA.
Chemphyschem. 2013 Jul 22;14(10):2288-94. doi: 10.1002/cphc.201300449. Epub 2013 Jun 21.
The development of an all-glass separation-based sensor using microdialysis coupled to microchip electrophoresis with amperometric detection is described. The system includes a flow-gated interface to inject discrete sample plugs from the microdialysis perfusate into the microchip electrophoresis system. Electrochemical detection was accomplished with a platinum electrode in an in-channel configuration using a wireless electrically isolated potentiostat. To facilitate bonding around the in-channel electrode, a fabrication process was employed that produced a working and a reference electrode flush with the glass surface. Both normal and reversed polarity separations were performed with this sensor. The system was evaluated in vitro for the continuous monitoring of the production of hydrogen peroxide from the reaction of glucose oxidase with glucose. Microdialysis experiments were performed using a BASi loop probe with an overall lag time of approximately five minutes and a rise time of less than 60 seconds.
本文描述了一种基于全玻璃分离的传感器的开发，该传感器采用微透析与微芯片电泳与安培检测相结合。该系统包括一个流量门接口，用于将微透析灌注液中的离散样品塞注入微芯片电泳系统。电化学检测采用通道内配置的铂电极和无线电隔离的电化学工作站进行。为了便于在通道内电极周围进行键合，采用了一种制造工艺，使工作电极和参比电极与玻璃表面齐平。该传感器可进行正常和反向极性分离。该系统在体外对葡萄糖氧化酶与葡萄糖反应产生的过氧化氢的连续监测进行了评估。微透析实验使用 BASi 环探头进行，总滞后时间约为 5 分钟，上升时间小于 60 秒。
