黑胡椒石油醚提取物的体外抗氧化活性。

In vitro antioxidant activity of pet ether extract of black pepper.

机构信息

Sri Sai College of Pharmacy, Badhani, Pathankot, Gurdaspur, India.

出版信息

Indian J Pharmacol. 2008 Aug;40(4):147-51. doi: 10.4103/0253-7613.43160.

DOI:10.4103/0253-7613.43160

PMID:20040947

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2792611/

Abstract

OBJECTIVE

To investigate the in vitro antioxidant activity of different fractions (R1, R2 and R3) obtained from pet ether extract of black pepper fruits (Piper nigrum Linn.)

MATERIALS AND METHODS

The fractions R1, R2 and R3 were eluted from pet ether and ethyl acetate in the ratio of 6:4, 5:5 and 4:6, respectively. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, superoxide anion radical, nitric oxide radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the extract.

RESULTS

The free radical scavenging activity of the different fractions of pet ether extract of P. nigrum (PEPN) increased in a concentration dependent manner. The R3 and R2 fraction of PEPN in 500 microg/ml inhibited the peroxidation of a linoleic acid emulsion by 60.48+/-3.33% and 58.89+/-2.51%, respectively. In DPPH free radical scavenging assay, the activity of R3 and R2 were found to be almost similar. The R3 (100microg/ml) fraction of PEPN inhibited 55.68+/-4.48% nitric oxide radicals generated from sodium nitroprusside, whereas curcumin in the same concentration inhibited 84.27+/-4.12%. Moreover, PEPN scavenged the superoxide radical generated by the Xanthine/Xanthine oxidase system. The fraction R2 and R3 in the doses of 1000microg/ml inhibited 61.04+/-5.11% and 63.56+/-4.17%, respectively. The hydroxyl radical was generated by Fenton's reaction. The amounts of total phenolic compounds were determined and 56.98 microg pyrocatechol phenol equivalents were detected in one mg of R3.

CONCLUSIONS

P. nigrum could be considered as a potential source of natural antioxidant.

摘要

目的

研究从黑胡椒果实（Piper nigrum Linn.）石油醚提取物中获得的不同馏分（R1、R2 和 R3）的体外抗氧化活性。

材料与方法

将 R1、R2 和 R3 分别用石油醚和乙酸乙酯以 6:4、5:5 和 4:6 的比例洗脱。进行 1,1-二苯基-2-苦基肼（DPPH）自由基、超氧阴离子自由基、一氧化氮自由基和羟自由基清除试验以评估提取物的抗氧化潜力。

结果

黑胡椒石油醚提取物（PEPN）的不同馏分的自由基清除活性呈浓度依赖性增加。在 500μg/ml 时，PEPN 的 R3 和 R2 馏分分别抑制亚油酸乳液的过氧化作用 60.48+/-3.33%和 58.89+/-2.51%。在 DPPH 自由基清除试验中，发现 R3 和 R2 的活性几乎相似。PEPN 的 R3（100μg/ml）馏分抑制 55.68+/-4.48%由硝普酸钠产生的一氧化氮自由基，而相同浓度的姜黄素抑制 84.27+/-4.12%。此外，PEPN 清除黄嘌呤/黄嘌呤氧化酶系统产生的超氧自由基。在 1000μg/ml 的剂量下，R2 和 R3 馏分分别抑制 61.04+/-5.11%和 63.56+/-4.17%。羟基自由基由 Fenton 反应产生。测定总酚类化合物的含量，在 1mg 的 R3 中检测到 56.98μg 邻苯二酚酚当量。