X射线衍射证据表明膜表面存在离散水层。

X-ray diffraction evidence for the presence of discrete water layers on the surface of membranes.

作者信息

Murthy N S, Worthington C R

机构信息

Department of Biological Sciences, Carnegie-Mellon University, Pittsburgh, PA.

出版信息

Biochim Biophys Acta. 1991 Feb 25;1062(2):172-6. doi: 10.1016/0005-2736(91)90389-p.

DOI:10.1016/0005-2736(91)90389-p

PMID:2004106

Abstract

X-ray diffraction spacings in multilayered membranes obtained from frog sciatic nerves were found to increase in discrete steps of approx. 5 A during swelling. These observed jumps in the repeat period suggest that the lipid bilayers exist in distinct states of hydration, and perhaps the swelling occurs by step-wise addition of water layers between the polar head groups. Our analysis and statistical tests of this hypothesis are presented.

摘要

研究发现，从青蛙坐骨神经获取的多层膜中的X射线衍射间距在肿胀过程中以约5埃的离散步长增加。这些在重复周期中观察到的跳跃表明脂质双层以不同的水合状态存在，并且肿胀可能是通过在极性头部基团之间逐步添加水层而发生的。本文给出了对该假设的分析和统计检验。