Mahida Vilsu I, Bhatti Apksha, Gupte Snehalata C
Surat Raktadan Kendra and Research Centre, Gopipura, Surat - 395 001, Gujarat, India.
Asian J Transfus Sci. 2008 Jan;2(1):9-12. doi: 10.4103/0973-6247.39504.
Our blood bank is a regional blood transfusion centre, which accepts blood only from voluntary donors.
The aim is to study iron status of regular voluntary donors who donated their blood at least twice in a year.
Prior to blood donation, blood samples of 220 male and 30 female voluntary donors were collected. Control included 100 each male and female healthy individuals in the 18- to 60-year age group, who never donated blood and did not have any chronic infection. In the study and control groups, about 10% subjects consumed non-vegetarian diet. After investigation, 85 males and 56 females having haemoglobin (Hb) levels above 12.5 g/dl were selected as controls. Donors were divided into </=10, 11-20, 21-50 and >50 blood donation categories. Majority of the donors in >50 donation category donated blood four times in a year, whereas the remaining donors donated two to three times per year. Haematological parameters were measured on fully automatic haematology analyzer, serum iron and total iron-binding capacity (TIBC) by biochemical methods, ferritin using ELISA kits and transferrin using immunoturbidometry kits. Iron/TIBC ratio x 100 gave percentage of transferrin saturation value.
Statistical evaluation was done by mean, standard deviation, pair t-test, chi(2) and anova (F-test).
Preliminary analysis revealed that there was no significant difference in the iron profile of vegetarian and non-vegetarian subjects or controls and the donors donating <20 times. Significant increase or decrease was observed in mean values of various haematological and iron parameters in donors who donated blood for >20 times (P < 0.001), compared to controls. Anaemia, iron deficiency and depletion of iron stores were more prevalent in female donors (P < 0.05) compared to males and especially in those male donors who donated their blood for more than 20 times.
Regular voluntary blood donors should receive iron supplementation to prevent iron deficiency and depletion in iron stores.
我们的血库是一个地区性输血中心，只接受自愿献血者的血液。
旨在研究每年至少献血两次的定期自愿献血者的铁状态。
在献血前，采集了220名男性和30名女性自愿献血者的血样。对照组包括100名年龄在18至60岁之间、从未献血且无任何慢性感染的健康男性和女性个体。在研究组和对照组中，约10%的受试者食用非素食。经过调查，选择了85名血红蛋白（Hb）水平高于12.5 g/dl的男性和56名女性作为对照组。献血者被分为献血次数≤10次、11 - 20次、21 - 50次和>50次四类。献血次数>50次的大多数献血者每年献血4次，而其余献血者每年献血2至3次。血液学参数在全自动血液分析仪上进行测量，血清铁和总铁结合力（TIBC）采用生化方法测定，铁蛋白使用ELISA试剂盒测定，转铁蛋白使用免疫比浊法试剂盒测定。铁/TIBC比值×100得出转铁蛋白饱和度百分比值。
采用均值、标准差、配对t检验、卡方检验和方差分析（F检验）进行统计评估。
初步分析显示，素食和非素食受试者或对照组与献血次数<20次的献血者的铁谱无显著差异。与对照组相比，献血次数>20次的献血者的各种血液学和铁参数的均值出现显著升高或降低（P < 0.001）。与男性相比，女性献血者中贫血、缺铁和铁储备耗竭更为普遍（P < 0.05），尤其是那些献血次数超过20次的男性献血者。
定期自愿献血者应接受铁补充剂以预防缺铁和铁储备耗竭。
