Unidad de Investigación, Fundación Hospital de Jove, Gijon, Spain.
J Cancer Res Clin Oncol. 2010 Jul;136(7):1049-58. doi: 10.1007/s00432-009-0750-x. Epub 2009 Dec 30.
To investigate the expression of matrix metalloproteases (MMPs) and their inhibitors (TIMPs) in patients who develop local recurrence (LR) after mastectomy.
We analyzed the expressions of MMP-1, -2, -7, -9, -11, -13, -14, TIMP-1, -2, and -3, using immunohistochemical techniques, in primary tumors from patients without tumoral recurrence (n = 50), patients who developed distant metastasis (n = 50), and from patients who develop LRs (n = 25). LRs of the latter group were also analyzed for MMPs expression. All the patients underwent mastectomy.
Score values for all MMPs and TIMPs were significantly higher in primary tumors of patients with distant metastasis. Primary tumors from patients with LR have lower expressions of MMPs and TIMPs compared with those from patients who developed distant metastasis, and with patients without recurrence for some MMPs. Remarkably, however, primary tumors from patients with LR showed significantly higher percentage of TIMP-1 and 2 expression in stromal cells compared to primary tumors from patients with distant metastasis or primary tumors from patients without tumoral progression. Furthermore, LRs had significantly higher MMP-9 expression than their corresponding primary tumors.
Our data indicate differences in MMPs/TIMPs expression between primary tumors of patients with LRs and of those with distant metastasis, both after mastectomy for breast cancer.
研究基质金属蛋白酶（MMPs）及其抑制剂（TIMPs）在乳腺癌根治术后发生局部复发（LR）的患者中的表达情况。
我们使用免疫组织化学技术分析了 50 例无肿瘤复发患者（对照组）、50 例发生远处转移患者和 25 例发生 LR 患者的原发肿瘤中 MMP-1、-2、-7、-9、-11、-13、-14、TIMP-1、-2 和 -3 的表达情况。对后者组的 LR 也进行了 MMPs 表达分析。所有患者均接受了乳腺癌根治术。
远处转移患者的原发肿瘤中所有 MMPs 和 TIMPs 的评分值均显著升高。LR 患者的原发肿瘤与发生远处转移的患者相比，以及与无肿瘤进展的患者相比，MMPs 和 TIMPs 的表达水平均较低，但对于某些 MMPs 则较低。然而，令人惊讶的是，LR 患者的原发肿瘤中基质细胞 TIMP-1 和 2 的表达百分比明显高于发生远处转移的患者或无肿瘤进展的患者。此外，LR 的 MMP-9 表达明显高于其相应的原发肿瘤。
我们的数据表明，LR 患者和发生远处转移的患者的 MMPs/TIMPs 表达存在差异，这两种患者均在乳腺癌根治术后。
