Vizoso F J, Rodriguez M, Altadill A, González-Diéguez M L, Linares A, González L O, Junquera S, Fresno-Forcelledo F, Corte M D, Rodrigo L
Unidad de Investigación, Fundación Hospital de Jove, Gijón, and Instituto Universitario de Oncología del Principado de Asturias, Oviedo, Spain.
World J Gastroenterol. 2007 Jun 21;13(23):3221-7. doi: 10.3748/wjg.v13.i23.3221.
To evaluate the tissular expression of Androgen (A), Estrogen (E) and Progesterone (Pg) receptors, and Apolipoprotein D (ApoD), in liver tumors from resected hepatocellular carcinoma (HCC) cases in order to assess their possible relationship to prognosis.
We performed an immunohistochemical study using tissue microarrays (containing more than 260 cancer specimens, from 31 HCC patients and controls) to determine the presence of specific antibodies against AR, ER, PgR and ApoD, correlating their findings with several clinico-pathological and biological variables. The staining results were categorized using a semi-quantitative score based on their intensity, and the percentage of immunostained cells was measured.
A total of 21 liver tumors (67.7%) were positive for AR; 16 (51.6%) for ER; 26 (83.9%) for PgR and 12 (38.7%) stained for ApoD. We have found a wide variability in the immunostaining score values for each protein, with a median (range) of 11.5 (11.5-229.5) for AR; 11.1 (8.5-65) for ER; 14.2 (4-61) for PgR; and 37.7 (13.8-81.1) for ApoD. A history of heavy ethanol consumption, correlated positively with AR and PgR and negatively with ER status. HCV chronic infection also correlated positively with AR and PgR status. However, the presence of ApoD immunostaining did not correlate with any of these variables. Tumors with a positive immuno-staining for PgR showed a better prognosis.
Our results indicate a moderate clinical value of the steroid receptor status in HCC, emphasizing the need to perform further studies in order to evaluate the possible role of new hormonal-based therapies.
评估雄激素（A）、雌激素（E）和孕激素（Pg）受体以及载脂蛋白D（ApoD）在肝细胞癌（HCC）手术切除病例肝脏肿瘤中的组织表达情况，以评估它们与预后的可能关系。
我们使用组织微阵列（包含来自31例HCC患者和对照的260多个癌症标本）进行免疫组织化学研究，以确定抗雄激素受体（AR）、雌激素受体（ER）、孕激素受体（PgR）和ApoD的特异性抗体的存在情况，并将研究结果与多个临床病理和生物学变量相关联。根据染色强度使用半定量评分对染色结果进行分类，并测量免疫染色细胞的百分比。
共有21例肝脏肿瘤（67.7%）AR呈阳性；16例（51.6%）ER呈阳性；26例（83.9%）PgR呈阳性；12例（38.7%）ApoD染色阳性。我们发现每种蛋白质的免疫染色评分值存在很大差异，AR的中位数（范围）为11.5（11.5 - 229.5）；ER为11.1（8.5 - 65）；PgR为14.2（4 - 61）；ApoD为37.7（13.8 - 81.1）。大量饮酒史与AR和PgR呈正相关，与ER状态呈负相关。丙型肝炎病毒（HCV）慢性感染也与AR和PgR状态呈正相关。然而，ApoD免疫染色的存在与这些变量均无相关性。PgR免疫染色阳性的肿瘤预后较好。
我们的结果表明类固醇受体状态在HCC中具有一定的临床价值，强调需要进行进一步研究以评估新的激素疗法的可能作用。
