间质金属蛋白酶在前列腺增生和前列腺癌患者中的表达比较研究。
Comparative study of stromal metalloproteases expression in patients with benign hyperplasia and prostate cancer.
机构信息
Servicio de Urología, Hospital de Jove, Avda. Eduardo Castro s/n, 33920, Gijón, Asturias, Spain.
出版信息
J Cancer Res Clin Oncol. 2011 Mar;137(3):551-5. doi: 10.1007/s00432-010-0906-8. Epub 2010 May 28.
PURPOSE
The aim of the present work was to perform a comparative study of stromal cell expressions of MMPs and TIMPs between benign and malignant prostate tissues.
METHODS
An immunohistochemical study was performed using specific antibodies against metalloproteases (MMPs) -1, -2, -7, 9, 11, 13, 14 and their tissue inhibitors (TIMPs) -1, 2 and 3, on prostate specimens from 133 patients with clinical localized prostate carcinoma and from 50 patients with BPH.
RESULTS
Our results showed higher percentages of expressions of MMPs and TIMPs by fibroblasts or by mononuclear inflammatory cells (MICs) in prostate carcinomas compared to these cells in BPH. The detection of MMP-2 expression by stromal fibroblasts and/or MMP-2, 9 and TIMP-3 expression by stromal MICs was associated with a 100% of specificity for diagnoses of prostate cancer. We found that the combination of MMP-2 expression by fibroblasts and/or MMP-9 by MICs and/or TIMP-2 by MICs yielded a sensitivity of 47.4%.
CONCLUSIONS
Despite of a limited sensitivity (50%), the combination of MMP-TIMPs expression in stromal cells (MMP-2 by fibroblasts and TIMPs by MICs) in our study provided a specificity of 100% for prostate cancer diagnosis.
目的
本研究旨在比较良性和恶性前列腺组织中基质细胞金属基质蛋白酶（MMPs）和组织抑制剂（TIMPs）的表达。
方法
采用针对 MMPs-1、-2、-7、9、11、13、14 及其组织抑制剂（TIMPs）-1、2 和 3 的特异性抗体，对 133 例临床局限性前列腺癌患者和 50 例 BPH 患者的前列腺标本进行免疫组织化学研究。
结果
我们的研究结果显示，与 BPH 相比，前列腺癌中基质细胞（成纤维细胞或单核炎性细胞[MICs]）中 MMPs 和 TIMPs 的表达比例更高。基质成纤维细胞表达 MMP-2 和/或基质 MICs 表达 MMP-2、9 和 TIMP-3 可特异性诊断前列腺癌（特异性 100%）。我们发现，成纤维细胞表达 MMP-2 和/或 MICs 表达 MMP-9 和/或 MICs 表达 TIMP-2 的组合敏感性为 47.4%。
结论
尽管敏感性有限（50%），但本研究中基质细胞（成纤维细胞的 MMP-2 和 MICs 的 TIMPs）中 MMP-TIMP 表达的组合对前列腺癌诊断的特异性为 100%。
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