Department of Food Science and Technology, Sejong University, Seoul, Republic of Korea.
J Med Food. 2009 Dec;12(6):1260-7. doi: 10.1089/jmf.2008.1289.
This study examined the effect of isoflavone-enriched milk on bone loss in ovariectomized (OVX) rats. Thirty 6-week-old Sprague-Dawley female rats were divided into two groups: sham-operated and OVX. The OVX group was subdivided into three dietary groups (OVX, non-isoflavone-enriched milk; OVX+Iso, isoflavone-enriched milk; and OVX+Iso+Vit+Ca, isoflavone-, vitamins D and K-, and Ca-enriched mik). After 19 weeks of feeding, the food efficiency ratio and body weight gain in the sham-operated group were significantly lower compared with those in the other groups. The bone alkaline phosphatase and total alkaline phosphatase activities were significantly higher in isoflavone-enriched groups (OVX+Iso and OVX+Iso+Vit+Ca) when compared with the sham-operated group. Urinary excretions of deoxypyridinoline and hydroxyproline were significantly higher with ovariectomy, but mostly normalized in the OVX+Iso and OVX+Iso+Vit+Ca groups. The rats in the OVX+Iso and OVX+Iso+Vit+Ca groups showed higher femur and tibia weights. A significant increase was found in bone density of femur and trabecular bone area in the OVX+Iso+Vit+Ca group, which almost reached that of the sham-operated group, whereas no difference was found among the OVX and OVX+Iso groups. The histological areas of the proximal tibia sections showed highly filled trabecular bone in both isoflavone-enriched groups (OVX+Iso and OVX+Iso+Vit+Ca). The present study indicated that isoflavone-enriched milk may have a partial preventive effect on ovariectomy-induced bone loss; however, vitamins D and K and Ca enrichment with isoflavone may enhance effectiveness for increasing bone mass in OVX rats.
本研究探讨了富含异黄酮的牛奶对去卵巢（OVX）大鼠骨丢失的影响。将 30 只 6 周龄的 Sprague-Dawley 雌性大鼠分为两组：假手术组和 OVX 组。OVX 组再分为三组饮食组（OVX、非异黄酮富集牛奶；OVX+Iso、异黄酮富集牛奶；OVX+Iso+Vit+Ca、异黄酮、维生素 D 和 K、钙富集奶）。19 周喂养后，假手术组的食物效率比和体重增加明显低于其他组。与假手术组相比，富含异黄酮组（OVX+Iso 和 OVX+Iso+Vit+Ca）的骨碱性磷酸酶和总碱性磷酸酶活性显著升高。去卵巢后，尿脱氧吡啶啉和羟脯氨酸排泄量显著升高，但 OVX+Iso 和 OVX+Iso+Vit+Ca 组基本正常。OVX+Iso 和 OVX+Iso+Vit+Ca 组的大鼠股骨和胫骨重量较高。OVX+Iso+Vit+Ca 组股骨和小梁骨面积的骨密度显著增加，几乎达到假手术组水平，而 OVX 和 OVX+Iso 组之间无差异。胫骨近端节段的组织学面积显示富含两种异黄酮的富钙组（OVX+Iso 和 OVX+Iso+Vit+Ca）的小梁骨高度填充。本研究表明，富含异黄酮的牛奶可能对去卵巢引起的骨丢失有一定的预防作用；然而，异黄酮与维生素 D 和 K 和钙的富集可能会增强对去卵巢大鼠增加骨量的效果。
