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Ruling out novel H1N1 influenza virus infection with direct fluorescent antigen testing.通过直接荧光抗原检测排除新型甲型H1N1流感病毒感染。
Clin Infect Dis. 2009 Sep 15;49(6):e66-8. doi: 10.1086/644502.
2
Rapid-test sensitivity for novel swine-origin influenza A (H1N1) virus in humans.新型猪源甲型流感（H1N1）病毒在人类中的快速检测敏感性。
N Engl J Med. 2009 Aug 13;361(7):728-9. doi: 10.1056/NEJMc0904264. Epub 2009 Jun 29.
3
Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak.纽约市疫情期间新型2009甲型流感（H1N1）检测多种测试方法的评估
J Clin Virol. 2009 Jul;45(3):191-5. doi: 10.1016/j.jcv.2009.06.005. Epub 2009 Jun 16.
4
Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients.将实时荧光定量聚合酶链反应与Binax NOW以及细胞离心涂片免疫荧光法用于检测住院患者的流感病毒进行比较。
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5
Comparison of flocked and rayon swabs for collection of respiratory epithelial cells from uninfected volunteers and symptomatic patients.植绒拭子与粘胶纤维拭子用于从未感染志愿者和有症状患者中采集呼吸道上皮细胞的比较。
J Clin Microbiol. 2006 Jun;44(6):2265-7. doi: 10.1128/JCM.02055-05.
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Comparison of SmartCycler real-time reverse transcription-PCR assay in a public health laboratory with direct immunofluorescence and cell culture assays in a medical center for detection of influenza A virus.公共卫生实验室中SmartCycler实时逆转录-聚合酶链反应检测法与医学中心直接免疫荧光法及细胞培养法在甲型流感病毒检测中的比较
J Clin Microbiol. 2003 Aug;41(8):3597-601. doi: 10.1128/JCM.41.8.3597-3601.2003.
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SimulFluor respiratory screen for rapid detection of multiple respiratory viruses in clinical specimens by immunofluorescence staining.SimulFluor呼吸道筛查试剂，用于通过免疫荧光染色快速检测临床标本中的多种呼吸道病毒。
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细胞离心增强免疫荧光法和样本质量对新型猪源（H1N1）流感病毒检测的影响。

Cytospin-enhanced immunofluorescence and impact of sample quality on detection of novel swine origin (H1N1) influenza virus.

机构信息

Clinical Virology Laboratory, Yale New Haven Hospital, and Department of Laboratory Medicine,Yale University School of Medicine, New Haven, Connecticut 06520-8035, USA.

出版信息

J Clin Microbiol. 2010 Mar;48(3):957-9. doi: 10.1128/JCM.01678-09. Epub 2009 Dec 30.

DOI:10.1128/JCM.01678-09
PMID:20042632
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2832425/
Abstract

Nasopharyngeal swabs (n = 601) from 278 adult and 323 pediatric patients were tested within 24 h of receipt by cytospin-enhanced direct immunofluorescence antibody testing (DFA) and real-time reverse transcriptase PCR (RT-PCR) using the CDC assay. Cytospin-enhanced DFA detected 230 (84.6%) of 272 swine influenza A PCR-positive results overall but 25 (92.6%) of 27 positive results in patients less than 5 years old and 208 (96.7%) of 215 positive samples with cycle threshold values of <26.

摘要

对 278 例成年患者和 323 例儿科患者的 601 份鼻咽拭子标本，在收到标本后 24 小时内，通过细胞离心增强直接免疫荧光抗体检测（DFA）和使用 CDC 检测方法的实时逆转录聚合酶链反应（RT-PCR）进行检测。细胞离心增强 DFA 总体上检测到 272 份猪流感 A PCR 阳性结果中的 230 份（84.6%），但在年龄小于 5 岁的 27 份阳性结果中检测到 25 份（92.6%），在循环阈值<26 的 215 份阳性样本中检测到 208 份（96.7%）。