细胞离心增强免疫荧光法和样本质量对新型猪源（H1N1）流感病毒检测的影响。
Cytospin-enhanced immunofluorescence and impact of sample quality on detection of novel swine origin (H1N1) influenza virus.
机构信息
Clinical Virology Laboratory, Yale New Haven Hospital, and Department of Laboratory Medicine,Yale University School of Medicine, New Haven, Connecticut 06520-8035, USA.
出版信息
J Clin Microbiol. 2010 Mar;48(3):957-9. doi: 10.1128/JCM.01678-09. Epub 2009 Dec 30.
Abstract
Nasopharyngeal swabs (n = 601) from 278 adult and 323 pediatric patients were tested within 24 h of receipt by cytospin-enhanced direct immunofluorescence antibody testing (DFA) and real-time reverse transcriptase PCR (RT-PCR) using the CDC assay. Cytospin-enhanced DFA detected 230 (84.6%) of 272 swine influenza A PCR-positive results overall but 25 (92.6%) of 27 positive results in patients less than 5 years old and 208 (96.7%) of 215 positive samples with cycle threshold values of <26.
摘要
对 278 例成年患者和 323 例儿科患者的 601 份鼻咽拭子标本，在收到标本后 24 小时内，通过细胞离心增强直接免疫荧光抗体检测（DFA）和使用 CDC 检测方法的实时逆转录聚合酶链反应（RT-PCR）进行检测。细胞离心增强 DFA 总体上检测到 272 份猪流感 A PCR 阳性结果中的 230 份（84.6%），但在年龄小于 5 岁的 27 份阳性结果中检测到 25 份（92.6%），在循环阈值<26 的 215 份阳性样本中检测到 208 份（96.7%）。
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