Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, China.
Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):52-7. doi: 10.1093/abbs/gmp102.
Hepatoma-derived growth factor (HDGF), a nuclear protein with both mitogenic and angiogenic activity, has been reported to be mainly involved in tumorigenesis and the progression of non-small cell lung cancer. In this study, the HDGF expression was knocked down by specific-shRNA with lentivirus expression vector targeting HDGF in lung squamous cell carcinoma 520 cells. HDGF knocked down by shRNA suppressed the cell proliferation significantly both in vitro and in vivo as indicated by MTT, plate clone and transplanted tumor model assays. In addition, the knocked-down expression of HDGF also inhibited cell migration and invasion as shown in transwell and Boyden experiments. We concluded that HDGF acts as an oncogene participating in the pathogenesis of squamous cell lung cancer, and HDGF may be a key therapeutic target for non-small cell lung cancer.
肝癌衍生生长因子（HDGF）是一种具有有丝分裂和血管生成活性的核蛋白，据报道主要参与肿瘤发生和非小细胞肺癌的进展。在这项研究中，通过靶向 HDGF 的慢病毒表达载体特异性短发夹 RNA（shRNA）敲低了肺鳞癌细胞 520 中的 HDGF 表达。MTT、平板克隆和移植瘤模型检测表明，shRNA 敲低 HDGF 显著抑制了细胞的体外和体内增殖。此外，HDGF 的敲低表达还抑制了细胞的迁移和侵袭，转染和 Boyden 实验结果表明了这一点。我们的结论是，HDGF 作为一种癌基因参与了鳞状细胞肺癌的发病机制，HDGF 可能是非小细胞肺癌的一个关键治疗靶点。
