Dutton A Q, Choong P F, Goh J C-H, Lee E H, Hui J H P
National University Health System, Singapore.
J Bone Joint Surg Br. 2010 Jan;92(1):169-75. doi: 10.1302/0301-620X.92B1.22629.
We hypothesised that meniscal tears treated with mesenchymal stem cells (MSCs) together with a conventional suturing technique would show improved healing compared with those treated by a conventional suturing technique alone. In a controlled laboratory study 28 adult pigs (56 knees) underwent meniscal procedures after the creation of a radial incision to represent a tear. Group 1 (n = 9) had a radial meniscal tear which was left untreated. In group 2 (n = 19) the incision was repaired with sutures and fibrin glue and in group 3, the experimental group (n = 28), treatment was by MSCs, suturing and fibrin glue. At eight weeks, macroscopic examination of group 1 showed no healing in any specimens. In group 2 no healing was found in 12 specimens and incomplete healing in seven. The experimental group 3 had 21 specimens with complete healing, five with incomplete healing and two with no healing. Between the experimental group and each of the control groups this difference was significant (p < 0.001). The histological and macroscopic findings showed that the repair of meniscal tears in the avascular zone was significantly improved with MSCs, but that the mechanical properties of the healed menisci remained reduced.
我们假设，与仅采用传统缝合技术治疗的半月板撕裂相比，采用间充质干细胞（MSC）联合传统缝合技术治疗的半月板撕裂愈合情况会更好。在一项对照实验室研究中，28只成年猪（56个膝关节）在制造一个放射状切口以模拟撕裂后接受了半月板手术。第1组（n = 9）有一个放射状半月板撕裂，未进行治疗。第2组（n = 19）的切口用缝线和纤维蛋白胶修复，第3组即实验组（n = 28），采用MSC、缝合和纤维蛋白胶进行治疗。8周时，对第1组的宏观检查显示，所有标本均未愈合。第2组中，12个标本未愈合，7个标本愈合不完全。实验组第3组有21个标本完全愈合，5个标本愈合不完全，2个标本未愈合。实验组与每个对照组之间的这种差异具有显著性（p < 0.001）。组织学和宏观检查结果显示，采用MSC可显著改善无血管区半月板撕裂的修复，但愈合半月板的力学性能仍有所降低。
