Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Norwida 25, Wroclaw, Poland.
J Ind Microbiol Biotechnol. 2010 May;37(5):431-5. doi: 10.1007/s10295-009-0687-8. Epub 2010 Jan 3.
An acetate negative mutant of Yarrowia lipolytica Wratislavia AWG7 was found to be suitable for the production of high amounts of citric acid in long-term repeated-batch cultures. When 40% of fresh replaced medium was fed, this strain produced 154 g l(-1), on average, which corresponded to a 0.78 g g(-1) yield and a productivity of 1.05 g l(-1) h(-1). The activity of the culture remained stable for more than 1,650 h, i.e., 16 cycles of the repeated-batch bioreactors.
一株脂肪假丝酵母（Yarrowia lipolytica Wratislavia AWG7）的醋酸盐阴性突变株，适合在长期重复分批培养中生产大量柠檬酸。当补料 40%的新鲜培养基时，该菌株平均生产 154 g/L，相当于 0.78 g/g 的产率和 1.05 g/L/h 的生产率。该培养物的活性稳定超过 1650 h，即重复分批生物反应器的 16 个循环。
