Southwest University for Nationalities, Chengdu, Sichuan, China.
Biochem Genet. 2010 Jun;48(5-6):418-27. doi: 10.1007/s10528-009-9322-7. Epub 2010 Jan 3.
This study investigates the molecular mechanism by which yaks (Bos grunniens) adapt to hypoxia based on lactate dehydrogenase (LDH). Three LDH1 variants of the yak were revealed in tissue extracts by electrophoresis, including LDH1-F, LDH1-M, and LDH1-S. Kinetic analysis using purified LDH1 variants showed that the yak LDH1-M variant exhibited a similar K (m) (NADH) and the same mobility on a gel as bovine LDH1, and the LDH1-F variant showed significant differences in K (m) values for NADH or pyruvate from the other two variants of yak LDH1 and bovine LDH1. Among the three muscles assayed, yak longissimus dorsi showed the highest LDH activity and the lowest malate dehydrogenase (MDH) activity; heart muscle was exactly the opposite. Our results suggest that the three LDH1 variants might play an important role in the adaptation to hypoxia.
本研究以乳酸脱氢酶（LDH）为基础，探讨了牦牛适应低氧的分子机制。通过电泳，从组织提取物中发现了三种牦牛 LDH1 变体，包括 LDH1-F、LDH1-M 和 LDH1-S。使用纯化的 LDH1 变体进行的动力学分析表明，牦牛 LDH1-M 变体的 NADH K（m）值和在凝胶上的迁移率与牛 LDH1 相似，而 LDH1-F 变体的 NADH 或丙酮酸 K（m）值与牦牛 LDH1 和牛 LDH1 的其他两种变体有显著差异。在所检测的三种肌肉中，牦牛背最长肌的 LDH 活性最高，苹果酸脱氢酶（MDH）活性最低；而心肌则恰恰相反。我们的研究结果表明，这三种 LDH1 变体可能在适应低氧中发挥重要作用。
