Cui Z D, Tristram D, LaScolea L J, Kwiatkowski T, Kopti S, Ogra P L
Department of Pediatrics, State University of New York, Children's Hospital of Buffalo 14222.
Infect Immun. 1991 Apr;59(4):1465-9. doi: 10.1128/iai.59.4.1465-1469.1991.
Groups of BALB/c mice were orally immunized with Chlamydia trachomatis serovar L2/434/Bu in order to characterize the nature and kinetics of the chlamydial antibody response in the cervix and other mucosal sites. These animals were subsequently challenged intravaginally to determine whether oral immunization offers protection against chlamydial antigen shedding in the genital tract. Following oral immunization, immunoglobulin A antibody activity was detected in the genital tract as well as other mucosal sites. Subsequent intravaginal challenges exhibited booster effects on preexisting antibody activity in the genital tract. Significant protection against challenge infection in the genital tract was observed by oral immunization. This was indicated by the absence of any chlamydial antigen shedding in cervical secretions. On the other hand, passively administered chlamydial-specific serum immunoglobulin G antibody did not significantly influence the course of cervical shedding of the organism and did not confer any protection against a subsequent intravaginal challenge. It is concluded that prior oral immunization can induce a secretory antibody response in the genital tract and provide protection against subsequent infection.
为了确定子宫颈和其他黏膜部位衣原体抗体反应的性质和动力学，将BALB/c小鼠分组，用沙眼衣原体血清型L2/434/Bu进行口服免疫。随后对这些动物进行阴道内攻毒，以确定口服免疫是否能预防生殖道衣原体抗原的脱落。口服免疫后，在生殖道以及其他黏膜部位检测到免疫球蛋白A抗体活性。随后的阴道内攻毒对生殖道中预先存在的抗体活性产生了增强作用。通过口服免疫观察到对生殖道攻毒感染有显著的保护作用。这表现为宫颈分泌物中未出现任何衣原体抗原脱落。另一方面，被动给予衣原体特异性血清免疫球蛋白G抗体对该生物体在宫颈的脱落过程没有显著影响，也不能对随后的阴道内攻毒提供任何保护。结论是，预先口服免疫可诱导生殖道产生分泌性抗体反应，并对随后的感染提供保护。
