Second Department of General Surgery, Qingdao University, Qingdao City, People's Republic of China.
Am J Med Sci. 2010 Feb;339(2):141-4. doi: 10.1097/MAJ.0b013e3181c20d01.
In this study, the effects of Stichopus japonicus acid mucopolysaccharide (SJAMP) on the apoptosis of the human hepatocellular carcinoma cell line HepG2 were examined. The underlying mechanism was investigated by determining the effect of SJAMP on the expression of Bcl-2 and nm23-H1 genes in HepG2 cells. In vitro cultured HepG2 cells were treated with different concentrations of SJAMP. The dimethylthiazol (MTT) assay was used to determine the inhibition of cell proliferation. Expression of Bcl-2 and nm23-H1 genes was determined by Western blot analysis. The results showed that SJAMP inhibited the proliferation of HepG2 cells in a time- and dose-dependent manner, SJAMP induced apoptosis in HepG2 cells, and SJAMP decreased the expression of Bcl-2 and increased the expression of nm23-H1. We conclude that SJAMP inhibits the proliferation of HepG2 cells by inducing apoptosis. These results provide a theoretical basis for the utilization of SJAMP as a potential antitumor component for the treatment of hepatocellular carcinoma.
在这项研究中，研究了海参酸性黏多糖（SJAMP）对人肝癌细胞系 HepG2 细胞凋亡的影响。通过测定 SJAMP 对 HepG2 细胞中 Bcl-2 和 nm23-H1 基因表达的影响，探讨了其作用机制。用不同浓度的 SJAMP 处理体外培养的 HepG2 细胞。采用二甲基噻唑（MTT）法测定细胞增殖抑制率。采用 Western blot 分析检测 Bcl-2 和 nm23-H1 基因的表达。结果表明，SJAMP 呈时间和剂量依赖性抑制 HepG2 细胞的增殖，SJAMP 诱导 HepG2 细胞凋亡，SJAMP 降低 Bcl-2 表达，增加 nm23-H1 表达。我们得出结论，SJAMP 通过诱导细胞凋亡抑制 HepG2 细胞的增殖。这些结果为将 SJAMP 作为治疗肝癌的潜在抗肿瘤成分提供了理论依据。
