• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis.应用实时 PCR 和高分辨率熔解分析快速鉴定和区分布鲁氏菌分离株。
J Clin Microbiol. 2010 Mar;48(3):697-702. doi: 10.1128/JCM.02021-09. Epub 2010 Jan 6.
2
Identification of Brucella spp. isolated from human brucellosis in Malaysia using high-resolution melt (HRM) analysis.使用高分辨率熔解曲线分析（HRM）鉴定马来西亚人间布鲁氏菌病分离出的布鲁氏菌属。
Diagn Microbiol Infect Dis. 2015 Apr;81(4):227-33. doi: 10.1016/j.diagmicrobio.2014.12.012. Epub 2015 Jan 7.
3
Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis.通过基于实时PCR的单核苷酸多态性（SNP）分析快速鉴定布鲁氏菌分离株至种水平。
BMC Microbiol. 2008 Jun 2;8:86. doi: 10.1186/1471-2180-8-86.
4
Validated 5' nuclease PCR assay for rapid identification of the genus Brucella.用于快速鉴定布鲁氏菌属的经过验证的5'核酸酶PCR检测法。
J Clin Microbiol. 2004 May;42(5):2261-3. doi: 10.1128/JCM.42.5.2261-2263.2004.
5
Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems.适用于传统PCR和实时PCR系统的羊种布鲁氏菌、牛种布鲁氏菌、猪种布鲁氏菌、绵羊附睾种布鲁氏菌、犬种布鲁氏菌和沙鼠种布鲁氏菌的新型鉴定与区分
J Microbiol Methods. 2008 Oct;75(2):375-8. doi: 10.1016/j.mimet.2008.07.002. Epub 2008 Jul 15.
6
Rapid multiplex PCR assay for the simultaneous detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis.用于同时检测布鲁氏菌属、流产布鲁氏菌、马耳他布鲁氏菌和猪布鲁氏菌的快速多重 PCR 检测方法。
J Microbiol Biotechnol. 2011 Jan;21(1):89-92. doi: 10.4014/jmb.1007.07051.
7
Review of detection of Brucella spp. by polymerase chain reaction.聚合酶链反应检测布鲁氏菌属的综述。
Croat Med J. 2010 Aug;51(4):306-13. doi: 10.3325/cmj.2010.51.306.
8
Evaluation of three polymerase chain reaction techniques for detection of Brucella DNA in peripheral human blood.三种聚合酶链反应技术检测人外周血中布鲁氏菌DNA的评估
Can J Microbiol. 2008 May;54(5):352-7. doi: 10.1139/w08-017.
9
Development of a PCR assay for typing and subtyping of Brucella species.建立一种用于布鲁氏菌属种分型和亚型分析的 PCR 检测方法。
Int J Med Microbiol. 2009 Dec;299(8):563-73. doi: 10.1016/j.ijmm.2009.05.002. Epub 2009 Jun 27.
10
Use of amplified fragment length polymorphism to identify and type Brucella isolates of medical and veterinary interest.使用扩增片段长度多态性来鉴定具有医学和兽医学意义的布鲁氏菌分离株并进行分型。
J Clin Microbiol. 2005 Feb;43(2):761-9. doi: 10.1128/JCM.43.2.761-769.2005.

引用本文的文献

1
Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis.通过高分辨率熔解分析快速监测 SARS-CoV-2 关注变体。
Sci Rep. 2023 Dec 7;13(1):21598. doi: 10.1038/s41598-023-48929-1.
2
First seroprevalence and molecular identification report of among dogs in Greater Cairo region and Damietta Governorate of Egypt.埃及大开罗地区和达米埃塔省犬类中[相关内容缺失]的首次血清流行率及分子鉴定报告。
Vet World. 2023 Jan;16(1):229-238. doi: 10.14202/vetworld.2023.229-238. Epub 2023 Jan 31.
3
Assays for Identification and Differentiation of Species: A Review.物种鉴定与区分的检测方法：综述
Microorganisms. 2022 Aug 6;10(8):1584. doi: 10.3390/microorganisms10081584.
4
High-Resolution Melting PCR as Rapid Genotyping Tool for Species.高分辨率熔解曲线PCR作为物种快速基因分型工具
Microorganisms. 2022 Feb 1;10(2):336. doi: 10.3390/microorganisms10020336.
5
MALDI-TOF mass spectrometry and high-resolution melting PCR for the identification of Mycoplasma bovis isolates.基质辅助激光解吸电离飞行时间质谱法和高分辨率熔解曲线聚合酶链反应用于鉴定牛支原体分离株。
BMC Vet Res. 2021 Apr 17;17(1):170. doi: 10.1186/s12917-021-02870-5.
6
Canine Brucellosis: An Update.犬布鲁氏菌病：最新进展
Front Vet Sci. 2021 Mar 2;8:594291. doi: 10.3389/fvets.2021.594291. eCollection 2021.
7
Direct detection of Corynebacterium striatum, Corynebacterium propinquum, and Corynebacterium simulans in sputum samples by high-resolution melt curve analysis.利用高分辨率熔解曲线分析直接检测痰标本中的棒状杆菌属、中间型棒状杆菌和相似棒状杆菌。
BMC Infect Dis. 2021 Jan 7;21(1):21. doi: 10.1186/s12879-020-05633-z.
8
Molecular characterization of Brucella species detected in humans and domestic ruminants of pastoral areas in Kagera ecosystem, Tanzania.坦桑尼亚卡盖拉生态系统中牧区人类和家养反刍动物中检出的布鲁氏菌种的分子特征。
Vet Med Sci. 2020 Nov;6(4):711-719. doi: 10.1002/vms3.298. Epub 2020 Jun 21.
9
Brucellosis in China: history, progress and challenge.中国布鲁氏菌病：历史、进展与挑战。
Infect Dis Poverty. 2020 May 24;9(1):55. doi: 10.1186/s40249-020-00673-8.
10
Three-reaction high-resolution melting assay for rapid differentiation of Mycobacterium tuberculosis complex members.三反应高分辨率熔解分析快速区分结核分枝杆菌复合群成员。
Microbiologyopen. 2019 Dec;8(12):e919. doi: 10.1002/mbo3.919. Epub 2019 Aug 25.

本文引用的文献

1
Brucella inopinata sp. nov., isolated from a breast implant infection.意外布鲁菌，新种，从乳房植入物感染中分离得到。
Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):801-808. doi: 10.1099/ijs.0.011148-0. Epub 2009 Aug 6.
2
Comparison of two multiple-locus variable-number tandem-repeat analysis methods for molecular strain typing of human Brucella melitensis isolates from the Middle East.两种多位点可变数目串联重复序列分析方法用于中东地区人源布鲁氏菌羊种分离株分子菌株分型的比较
J Clin Microbiol. 2009 Jul;47(7):2226-31. doi: 10.1128/JCM.02362-08. Epub 2009 May 13.
3
Rapid identification of multidrug-resistant Mycobacterium tuberculosis isolates by rpoB gene scanning using high-resolution melting curve PCR analysis.利用高分辨率熔解曲线PCR分析通过rpoB基因扫描快速鉴定耐多药结核分枝杆菌分离株
J Antimicrob Chemother. 2009 Jun;63(6):1121-7. doi: 10.1093/jac/dkp124. Epub 2009 Apr 15.
4
Genotyping of Chlamydophila psittaci by real-time PCR and high-resolution melt analysis.通过实时聚合酶链反应和高分辨率熔解分析对鹦鹉热嗜衣原体进行基因分型。
J Clin Microbiol. 2009 Jan;47(1):175-81. doi: 10.1128/JCM.01851-08. Epub 2008 Nov 12.
5
Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems.适用于传统PCR和实时PCR系统的羊种布鲁氏菌、牛种布鲁氏菌、猪种布鲁氏菌、绵羊附睾种布鲁氏菌、犬种布鲁氏菌和沙鼠种布鲁氏菌的新型鉴定与区分
J Microbiol Methods. 2008 Oct;75(2):375-8. doi: 10.1016/j.mimet.2008.07.002. Epub 2008 Jul 15.
6
Detection of macrolide resistance in Mycoplasma pneumoniae by real-time PCR and high-resolution melt analysis.通过实时聚合酶链反应和高分辨率熔解分析检测肺炎支原体中的大环内酯类耐药性。
Antimicrob Agents Chemother. 2008 Oct;52(10):3542-9. doi: 10.1128/AAC.00582-08. Epub 2008 Jul 21.
7
Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis.通过基于实时PCR的单核苷酸多态性（SNP）分析快速鉴定布鲁氏菌分离株至种水平。
BMC Microbiol. 2008 Jun 2;8:86. doi: 10.1186/1471-2180-8-86.
8
Brucella microti sp. nov., isolated from the common vole Microtus arvalis.微小布鲁氏菌新种，从普通田鼠（Microtus arvalis）中分离得到。
Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):375-82. doi: 10.1099/ijs.0.65356-0.
9
Real-time PCR assays of single-nucleotide polymorphisms defining the major Brucella clades.用于定义主要布鲁氏菌进化枝的单核苷酸多态性的实时聚合酶链反应分析
J Clin Microbiol. 2008 Jan;46(1):296-301. doi: 10.1128/JCM.01496-07. Epub 2007 Nov 21.
10
Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts.布鲁氏菌属新种布鲁氏菌鲸种和布鲁氏菌海豹种，其为以鲸类和海豹为主要宿主的布鲁氏菌菌株。
Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2688-2693. doi: 10.1099/ijs.0.65269-0.

应用实时 PCR 和高分辨率熔解分析快速鉴定和区分布鲁氏菌分离株。

Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis.

机构信息

National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

出版信息

J Clin Microbiol. 2010 Mar;48(3):697-702. doi: 10.1128/JCM.02021-09. Epub 2010 Jan 6.

DOI:10.1128/JCM.02021-09
PMID:20053863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2832424/
Abstract

Definitive identification of Brucella species remains a challenge due to the high degree of genetic homology shared within the genus. We report the development of a molecular technique which utilizes real-time PCR followed by high-resolution melt (HRM) curve analysis to reliably type members of this genus. Using a panel of seven primer sets, we tested 153 Brucella spp. isolates with >99% accuracy compared to traditional techniques. This assay provides a useful diagnostic tool that can rapidly type Brucella isolates and has the potential to detect novel species. This approach may also prove helpful for clinical, epidemiological and veterinary investigations.

摘要

由于属内具有高度的遗传同源性，因此对布鲁氏菌种的明确鉴定仍然是一个挑战。我们报告了一种分子技术的发展，该技术利用实时 PCR 结合高分辨率熔解（HRM）曲线分析可靠地区分该属的成员。使用一组七个引物对，我们对 153 株布鲁氏菌属菌株进行了测试，与传统技术相比，准确率超过 99%。该检测方法提供了一种有用的诊断工具，可快速对布鲁氏菌属菌株进行分型，并有可能检测到新的物种。这种方法对于临床、流行病学和兽医调查也可能证明是有用的。