National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 2010 Mar;48(3):697-702. doi: 10.1128/JCM.02021-09. Epub 2010 Jan 6.
Definitive identification of Brucella species remains a challenge due to the high degree of genetic homology shared within the genus. We report the development of a molecular technique which utilizes real-time PCR followed by high-resolution melt (HRM) curve analysis to reliably type members of this genus. Using a panel of seven primer sets, we tested 153 Brucella spp. isolates with >99% accuracy compared to traditional techniques. This assay provides a useful diagnostic tool that can rapidly type Brucella isolates and has the potential to detect novel species. This approach may also prove helpful for clinical, epidemiological and veterinary investigations.
由于属内具有高度的遗传同源性,因此对布鲁氏菌种的明确鉴定仍然是一个挑战。我们报告了一种分子技术的发展,该技术利用实时 PCR 结合高分辨率熔解(HRM)曲线分析可靠地区分该属的成员。使用一组七个引物对,我们对 153 株布鲁氏菌属菌株进行了测试,与传统技术相比,准确率超过 99%。该检测方法提供了一种有用的诊断工具,可快速对布鲁氏菌属菌株进行分型,并有可能检测到新的物种。这种方法对于临床、流行病学和兽医调查也可能证明是有用的。