Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium.
Biofouling. 2010;26(3):269-75. doi: 10.1080/08927010903501908.
In order to prevent biofilm formation by Candida albicans, several cationic peptides were covalently bound to polydimethylsiloxane (PDMS). The salivary peptide histatin 5 and two synthetic variants (Dhvar 4 and Dhvar 5) were used to prepare peptide functionalized PDMS using 4-azido-2,3,5,6-tetrafluoro-benzoic acid (AFB) as an interlinkage molecule. In addition, polylysine-, polyarginine-, and polyhistidine-PDMS surfaces were prepared. Dhvar 4 functionalized PDMS yielded the highest reduction of the number of C. albicans biofilm cells in the Modified Robbins Device. Amino acid analysis demonstrated that the amount of peptide immobilized on the modified disks was in the nanomole range. Poly-d-lysine PDMS, in particular the homopeptides with low molecular weight (2500 and 9600) showed the highest activity against C. albicans biofilms, with reductions of 93% and 91%, respectively. The results indicate that the reductions are peptide dependent.
为了防止白色念珠菌生物膜的形成，几种阳离子肽被共价结合到聚二甲基硅氧烷（PDMS）上。唾液肽Histatin 5 和两种合成变体（Dhvar 4 和 Dhvar 5）被用于使用 4-叠氮-2,3,5,6-四氟苯甲酸（AFB）作为连接分子来制备肽功能化 PDMS。此外，还制备了聚赖氨酸、聚精氨酸和聚组氨酸 PDMS 表面。Dhvar 4 功能化 PDMS 在改良的 Robbins 装置中产生了最高数量的白色念珠菌生物膜细胞的减少。氨基酸分析表明，固定在修饰的圆盘上的肽的量处于纳摩尔范围内。聚-d-赖氨酸 PDMS，特别是具有低分子量（2500 和 9600）的同型肽对白色念珠菌生物膜表现出最高的活性，分别降低了 93%和 91%。结果表明，减少与肽有关。
