National Institute for Research and Development in Microtechnology (IMT-Bucharest), Erou Iancu Nicolae 126 A, 72996, Bucharest, Romania.
J Biomed Nanotechnol. 2009 Jun;5(3):300-9. doi: 10.1166/jbn.2009.1035.
Emerging applications of porous silicon (PS) lies in its ability to incorporate other materials, such as organic groups, organic and inorganic nanoparticles to form (bio)hybrid systems where each individual constituent may be optimized for a particular function. This paper presents our recent experimental results on the fabrication and applications in biosensing of the porous silicon (PS) based microstructures. We have demonstrated that different morphologies of PS, either as-prepared or coated with gold nanoparticles have an important role in biomolecule detection, due to its large internal surface combined with specific electro-optical properties, being in the same time support for immobilization of complementary biomolecules as well as transducer for biochemical interactions. Therefore, we have investigated the photoluminescence properties of nanoporous Si prepared on different Si micropatterned surfaces comparatively with PS/flat Si in order to develop a new simple and versatile process for biosensor transducer fabrication. Meso- and macro-PS have been investigated for protein immobilization and detection using microarray technique or for DNA biomolecule detection by impedance spectroscopy. Finally, we have demonstrated that macroporous silicon constitutes an appropriate substrate for very sensitive SERS biosensors. RAMAN signal of 11-mercaptoundecanoic acid was investigated on Au/macroporous silicon. Various characterisation techniques have been used, optical and scanning electron microscopy (SEM) to investigate samples morphology, X-ray diffraction for nanoparticle structure, Raman and PL spectroscopy, and laser fluorescence detection for chemical and optical properties analysis and impedance spectroscopy for investigation organic molecule attachment on the Au/PS structures.
多孔硅（PS）的新兴应用在于其能够掺入其他材料，例如有机基团、有机和无机纳米粒子，从而形成（生物）杂化系统，其中每个单独的成分都可以针对特定功能进行优化。本文介绍了我们最近在基于多孔硅（PS）的微结构的生物传感方面的制造和应用的实验结果。我们已经证明，不同形态的 PS，无论是未经处理的还是涂覆有金纳米粒子的 PS，由于其大的内部表面积与特定的光电特性相结合，在生物分子检测中都具有重要作用，同时也是固定互补生物分子的支撑物以及生物化学相互作用的传感器。因此，我们比较了在不同 Si 微图案化表面上制备的纳米多孔 Si 的光致发光特性和 PS/平坦 Si，以便开发用于生物传感器换能器制造的新的简单且通用的工艺。介孔和大孔 PS 已被用于使用微阵列技术进行蛋白质固定和检测，或用于通过阻抗谱进行 DNA 生物分子检测。最后，我们证明了大孔硅是非常灵敏的 SERS 生物传感器的合适衬底。研究了金/大孔硅上 11-巯基十一酸的拉曼信号。使用各种表征技术，包括光学和扫描电子显微镜（SEM）来研究样品形态、X 射线衍射来研究纳米粒子结构、拉曼和 PL 光谱以及激光荧光检测来分析化学和光学性质，以及阻抗谱来研究有机分子在 Au/PS 结构上的附着。
