Sun Hanwen, Su Ming, Li Liqing
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding, 071002, China.
J Chromatogr Sci. 2010 Jan;48(1):49-54. doi: 10.1093/chromsci/48.1.49.
A simple, fast, and sensitive capillary electrophoresis method was developed for the simultaneous determination of tetracaine, proline, and enoxacin in human urine with electrochemiluminescence (ECL) detection. The effects of experimental conditions including the mode of applied voltage signal, the potential of working electrode, pH value, the flow rate of carrier of solution, the concentration of Ru(bpy)(3)(2+), and the ECL intensity of the drugs were investigated in detail. Parameters related to the separation and detection were investigated and optimized. Under optimized conditions, the proposed method displayed a linear range from 0.4 to 100 microg/mL for tetracaine, 0.2 to 80 microg/mL for proline, and 0.1 to 100 microg/mL for enoxacin. Their limits of detection were 0.08, 0.06, and 0.02 microg/mL, respectively. A baseline separation for tetracaine, proline, and enoxacin was achieved within 10 min. Developed method was successfully applied to determine tetracaine, proline, and enoxacin in human urine.
建立了一种简单、快速、灵敏的毛细管电泳方法，用于同时测定人尿中的丁卡因、脯氨酸和依诺沙星，并采用电化学发光（ECL）检测。详细研究了包括施加电压信号模式、工作电极电位、pH值、溶液载体流速、Ru(bpy)(3)(2+)浓度以及药物的ECL强度等实验条件的影响。对与分离和检测相关的参数进行了研究和优化。在优化条件下，该方法对丁卡因的线性范围为0.4至100μg/mL，对脯氨酸为0.2至80μg/mL，对依诺沙星为0.1至100μg/mL。它们的检测限分别为0.08、0.06和0.02μg/mL。在10分钟内实现了丁卡因、脯氨酸和依诺沙星的基线分离。所建立的方法成功应用于人尿中丁卡因、脯氨酸和依诺沙星的测定。
