Department of Immunology, Landspitali-University Hospital, Reykjavik, Iceland.
Vaccine. 2010 Feb 25;28(9):2097-103. doi: 10.1016/j.vaccine.2009.12.039. Epub 2010 Jan 5.
The influence of age on the generation and persistence of specific memory B cells after vaccination with Neisseria meningitidis type C polysaccharide (MenC-PS) conjugate is unknown. MenC-PS-specific B cells could be directly enumerated by fluorochrome-labeled MenC-PS and flow cytometry in blood up to at least 4 years after vaccination, ranging from 0.01% to 0.78% of total B cells and did not correlate with age at vaccination. The percentage of MenC-specific memory B cells out of total memory B cells correlated with total MenC-specific B-cells and with frequencies of IgA(+) plus IgG(+) MenC-specific AbSC, but not with MenC-specific Ab.
年龄对接种脑膜炎奈瑟菌 C 型多糖（MenC-PS）结合疫苗后特定记忆 B 细胞的产生和持续存在的影响尚不清楚。在接种疫苗后至少 4 年内，通过荧光标记的 MenC-PS 和流式细胞术可以直接计数 MenC-PS 特异性 B 细胞，范围从总 B 细胞的 0.01%到 0.78%，与接种时的年龄无关。MenC 特异性记忆 B 细胞在总记忆 B 细胞中的百分比与总 MenC 特异性 B 细胞以及 IgA(+)加 IgG(+)MenC 特异性 AbSC 的频率相关，但与 MenC 特异性 Ab 无关。
