Romeu Belkis, Lastre Miriam, García Luis, Cedré Bárbara, Mandariote Aleida, Fariñas Mildrey, Oliva Reynaldo, Rosenqvist Einar, Pérez Oliver
Immunology Department, Vice presidency of Research and Development, Havana, Cuba,
Immunol Res. 2014 Jan;58(1):75-85. doi: 10.1007/s12026-013-8427-6.
Outer-membrane vesicles (OMVs) have inherent adjuvant properties, and many vaccines use OMV as vaccine components. Utilizing the adjuvant properties of OMV could lead to the formulation of vaccines that are less expensive and potentially more immunogenic than covalently conjugated polysaccharide vaccines. We evaluated the adjuvant effect in Balb/c mice of combinations of OMV from Neisseria meningitidis serogroup A and W135 as compared to that of the non-covalently conjugated capsular polysaccharide A. Both antigens were adsorbed onto aluminum hydroxide. The mice were given a booster dose of plain polysaccharide A to stimulate an immunologic memory response. Subclasses determination and cytokine assays demonstrated the capacity of OMV to induce a IgG2a/IgG2b isotype profile and IFN-γ production, suggesting the induction of a Th1 pattern immune response. Lymphoproliferative responses to OMVs were high, with affinity maturation of antibodies observed. Bactericidal titers after the booster dose were also observed. Memory B cells and long-term memory T cells were also detected. The results of this study indicate that combined meningococcal serogroup A and W135 OMV can activate cell-mediated immunity and induce a long-term memory response.
外膜囊泡（OMV）具有内在的佐剂特性，许多疫苗使用OMV作为疫苗成分。利用OMV的佐剂特性可能会开发出比共价结合多糖疫苗更便宜且可能更具免疫原性的疫苗。我们评估了来自A群和W135群脑膜炎奈瑟菌的OMV组合在Balb/c小鼠中的佐剂效果，并与非共价结合的荚膜多糖A进行了比较。两种抗原均吸附于氢氧化铝上。给小鼠接种一剂普通多糖A加强针以刺激免疫记忆反应。亚类测定和细胞因子检测表明，OMV能够诱导IgG2a/IgG2b同型谱和IFN-γ产生，提示诱导了Th1型免疫反应。对OMV的淋巴细胞增殖反应较高，观察到抗体亲和力成熟。加强针接种后的杀菌效价也被观察到。还检测到记忆B细胞和长期记忆T细胞。本研究结果表明，A群和W135群脑膜炎球菌联合OMV可激活细胞介导的免疫并诱导长期记忆反应。
