Department of Developmental Biology and JST, CREST, Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan.
Biochem Biophys Res Commun. 2010 Feb 12;392(3):301-6. doi: 10.1016/j.bbrc.2009.12.180. Epub 2010 Jan 6.
FAM3C is a secreted factor, which is involved in the epithelial to mesenchymal transition. In transcriptome profiling of the mouse retina using microarray, we found that FAM3C is highly expressed in the retina. FAM3C is expressed in the ganglion cell layer (GCL) of the retina. To explore the function of FAM3C in retinal development, we cloned Xenopus FAM3C (XFAM3C), and performed Xenopus gain- and loss-of-function analysis. Overexpression of XFAM3C resulted in retinal laminar disorganization and an increase in eye size. Loss of function experiments of XFAM3C using antisense morpholino oligonucleotides caused photoreceptor cell dislocation. Cellular differentiation was not affected by either gain- or loss-of-function experiments of XFAM3C. These findings suggest that FAM3C is involved in retinal laminar formation processes in vertebrates.
FAM3C 是一种分泌因子，参与上皮间质转化。在使用微阵列对小鼠视网膜的转录组进行分析时，我们发现 FAM3C 在视网膜中高度表达。FAM3C 在视网膜的神经节细胞层（GCL）中表达。为了探究 FAM3C 在视网膜发育中的功能，我们克隆了非洲爪蟾 FAM3C（XFAM3C），并进行了非洲爪蟾的功能获得和功能丧失分析。XFAM3C 的过表达导致视网膜层状结构紊乱和眼睛增大。使用反义寡核苷酸的 XFAM3C 功能丧失实验导致光感受器细胞移位。细胞分化不受 XFAM3C 的功能获得或丧失实验的影响。这些发现表明 FAM3C 参与了脊椎动物视网膜层形成过程。
