Zhang Jinyang, Song Wenhua, Guo Jing, Zhang Jinhua, Sun Zengtian, Li Liying, Ding Feng, Gao Minling
College of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai, China.
Toxicol Ind Health. 2013 Jul;29(6):523-33. doi: 10.1177/0748233712442708. Epub 2012 Apr 16.
With large-scale production and wide application of nano-titanium oxide (TiO2), its health hazard has attracted extensive attention worldwide. In this study, mouse macrophages (Ana-1 and MH-S cells) were used to evaluate the cytotoxicity of different sized TiO2 nanoparticles. The results showed that TiO2 nanoparticles caused low toxicity, especially in MH-S cells. There was a difference in the cytotoxicity induced by different sized TiO2 particles. The 25 nm anatase particles induced the strongest cytotoxicity and oxidative stress, followed by 5 and 100 nm anatase particles; in contrast, 100 nm rutile particles induced the lowest toxicity. Although TiO2 nanoparticles induced high levels of intracellular reactive oxygen species (ROS), the determination of ROS demonstrated that the inherent oxidative capacity of TiO2 nanoparticles was lower in the absence of photoactivation. Therefore, the generation of intracellular ROS could not completely depend on inherent oxidative capacity of TiO2 nanoparticles. Toxicity of TiO2 nanoparticles could mainly depend on the structural characteristics.
随着纳米二氧化钛（TiO₂）的大规模生产和广泛应用，其对健康的危害已引起全球广泛关注。在本研究中，使用小鼠巨噬细胞（Ana-1和MH-S细胞）评估不同尺寸TiO₂纳米颗粒的细胞毒性。结果表明，TiO₂纳米颗粒引起的毒性较低，尤其是在MH-S细胞中。不同尺寸的TiO₂颗粒诱导的细胞毒性存在差异。25nm锐钛矿颗粒诱导的细胞毒性和氧化应激最强，其次是5nm和100nm锐钛矿颗粒；相比之下，100nm金红石颗粒诱导的毒性最低。尽管TiO₂纳米颗粒诱导了高水平的细胞内活性氧（ROS），但ROS的测定表明，在没有光激活的情况下，TiO₂纳米颗粒的固有氧化能力较低。因此，细胞内ROS的产生不能完全依赖于TiO₂纳米颗粒的固有氧化能力。TiO₂纳米颗粒的毒性可能主要取决于其结构特征。
