用于分离烟酰胺腺嘌呤二核苷酸磷酸特异性谷氨酸脱氢酶缺陷型脉孢菌突变体的直接选择程序。
Direct selective procedure for isolating Neurospora mutants defective in nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase.
作者信息
Kinsey J A
出版信息
J Bacteriol. 1977 Dec;132(3):751-6. doi: 10.1128/jb.132.3.751-756.1977.
Abstract
A procedure has been developed for isolating nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase (am) mutants of Neurospora. Physiological, genetic, and enzymatic tests show that the new mutants are am alleles. Reconstruction tests and analysis of the new alleles show that the procedure yields a broad spectrum of lesions at the am locus. The isolation of am mutants by this technique appears to be related to the effect of am mutants on the control of the general permease.
摘要
已开发出一种用于分离粗糙脉孢菌烟酰胺腺嘌呤二核苷酸磷酸特异性谷氨酸脱氢酶（am）突变体的方法。生理、遗传和酶学测试表明，新的突变体是am等位基因。对新等位基因的重建测试和分析表明，该方法在am位点产生了广泛的损伤。通过这种技术分离am突变体似乎与am突变体对一般通透酶控制的影响有关。
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Direct selective procedure for isolating Neurospora mutants defective in nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase.用于分离烟酰胺腺嘌呤二核苷酸磷酸特异性谷氨酸脱氢酶缺陷型脉孢菌突变体的直接选择程序。
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本文引用的文献
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Multiple active varieties of Neurospora glutamate dehydrogenase formed by hybridization between two inactive mutant proteins in vivo and in vitro.通过体内和体外两种无活性突变蛋白杂交形成的多个活性神经孢霉谷氨酸脱氢酶变体。
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Glutamic dehydrogenase in revertants of am mutants in Neurospora.粗糙脉孢菌am突变体回复突变体中的谷氨酸脱氢酶
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