用于分离烟酰胺腺嘌呤二核苷酸磷酸特异性谷氨酸脱氢酶缺陷型脉孢菌突变体的直接选择程序。
Direct selective procedure for isolating Neurospora mutants defective in nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase.
作者信息
Kinsey J A
出版信息
J Bacteriol. 1977 Dec;132(3):751-6. doi: 10.1128/jb.132.3.751-756.1977.
Abstract
A procedure has been developed for isolating nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase (am) mutants of Neurospora. Physiological, genetic, and enzymatic tests show that the new mutants are am alleles. Reconstruction tests and analysis of the new alleles show that the procedure yields a broad spectrum of lesions at the am locus. The isolation of am mutants by this technique appears to be related to the effect of am mutants on the control of the general permease.
摘要
已开发出一种用于分离粗糙脉孢菌烟酰胺腺嘌呤二核苷酸磷酸特异性谷氨酸脱氢酶（am）突变体的方法。生理、遗传和酶学测试表明，新的突变体是am等位基因。对新等位基因的重建测试和分析表明，该方法在am位点产生了广泛的损伤。通过这种技术分离am突变体似乎与am突变体对一般通透酶控制的影响有关。
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本文引用的文献
1
COMPLEMENTATION RELATIONSHIP OF NEUROSPORA AM MUTANTS IN RELATION TO THEIR FORMATION OF ABNORMAL VARIETIES OF GLUTAMATE DEHYDROGENASE.
Genet Res. 1965 Feb;6:121-9. doi: 10.1017/s0016672300003980.
2
MUTATIONAL ALTERATION OF PERMEABILITY IN NEUROSPORA: EFFECTS ON GROWTH AND THE UPTAKE OF CERTAIN AMINO ACIDS AND RELATED COMPOUNDS.
Genetics. 1964 Dec;50(6):1383-402. doi: 10.1093/genetics/50.6.1383.
3
IMMUNOLOGICAL STUDIES OF AMINATION DEFICIENT STRAINS OF NEUROSPORA CRASSIA.
J Gen Microbiol. 1964 Feb;34:295-305. doi: 10.1099/00221287-34-2-295.
4
Purification and characterization of a TPN-specific glutamic acid dehydrogenase Neurospora crassa.
Arch Biochem Biophys. 1963 Jul;102:66-76. doi: 10.1016/0003-9861(63)90321-7.
5
Suppressors of amino acid uptake mutants of Neurospora.
Genetics. 1967 Dec;57(4):935-42. doi: 10.1093/genetics/57.4.935.
6
Multiple active varieties of Neurospora glutamate dehydrogenase formed by hybridization between two inactive mutant proteins in vivo and in vitro.
J Mol Biol. 1966 Jun;17(2):503-12. doi: 10.1016/s0022-2836(66)80160-2.
7
Glutamic dehydrogenase in revertants of am mutants in Neurospora.
Genet Res. 1966 Feb;7(1):18-31. doi: 10.1017/s0016672300009447.
8
Reversion of the am locus in Neurospora: evidence for nonsense suppression.
Genetics. 1968 Jan;58(1):85-99. doi: 10.1093/genetics/58.1.85.
9
Amino acid transport in Neurospora crassa. II. Properties of a basic amino acid transport system.
Biochim Biophys Acta. 1970 Mar 17;203(1):139-49. doi: 10.1016/0005-2736(70)90044-1.
10
Ammonia inhibition of the general amino acid permease and its suppression in NADPH-specific glutamate dehydrogenaseless mutants of saccharomyces cerevisiae.
Biochem Biophys Res Commun. 1972 Aug 21;48(4):749-56. doi: 10.1016/0006-291x(72)90670-5.
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