采用傅里叶变换红外光谱（FTIR）、紫外光谱（UV）和气相色谱-质谱联用（GC-MS）分析菲的生物降解。
Analysis of phenanthrene biodegradation by using FTIR, UV and GC-MS.
机构信息
Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Mar;75(3):1047-50. doi: 10.1016/j.saa.2009.12.051. Epub 2009 Dec 22.
The aim of this paper is to assess the biodegradation of phenanthrene by Flavobacteria FCN2 which was isolated from coke plant sludge via a classical shaken liquid medium enrichment method. The strain FCN2 can decompose phenanthrene (50 mg l(-1)) completely within 5 days. The values of pH decrease to 6.7 from 7.2 during degradation periods. And a detailed phenanthrene metabolism was assayed by using FTIR, UV and GC-MS. For FTIR, appearance of new broad absorption bands at 2858 cm(-1), 2927 cm(-1), 2955 cm(-1) and another new strong absorption band at 1734 cm(-1) in metabolites demonstrates that carboxyl group produced during phenanthrene degradation. Besides this, a very strong absorption band appears at 1260 cm(-1). It is ascribed to C-C stretching vibration band in carbonyl group of arone. Two weak adsorption at 334 nm and 349 nm in UV spectra were assigned to the n-pi* transition of CO of aldehyde. Two metabolites, phenanthrene-dihydrodiol and naphthalene-1-diol were identified in neutral fraction of phenanthrene degradation by using GC-MS. As a result carboxylic acids and arone were generated during biodegradation of phenanthrene by Flavobacteria FCN2.
本文旨在评估从焦化厂污泥中通过经典的摇瓶液体培养基富集法分离得到的黄杆菌 FCN2 对菲的生物降解能力。该菌株 FCN2 可以在 5 天内完全降解 50mg/L 的菲。在降解过程中，pH 值从 7.2 下降到 6.7。通过使用傅里叶变换红外光谱（FTIR）、紫外光谱（UV）和气相色谱-质谱联用（GC-MS）详细研究了菲的代谢途径。对于 FTIR，在代谢物中出现了新的宽吸收带，在 2858cm(-1)、2927cm(-1)、2955cm(-1)和 1734cm(-1)处出现新的强吸收带，表明在菲降解过程中产生了羧基。此外，在 1260cm(-1)处出现了一个非常强的吸收带，它归因于芳酮羰基中的 C-C 伸缩振动带。在 UV 光谱中，在 334nm 和 349nm 处出现了两个较弱的吸附峰，这归因于醛的 CO 的 n-π*跃迁。通过 GC-MS 在菲降解中性部分鉴定出两种代谢物，即菲-二氢二醇和萘-1-二醇。结果表明，黄杆菌 FCN2 在降解菲的过程中生成了羧酸和芳酮。
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