Jiang Yiming, Huang Haiying, Wu Mengru, Yu Xuan, Chen Yong, Liu Pu, Li Xiangkai
Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Lanzhou, 730000 China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China.
Biophys Rep. 2015;1:156-167. doi: 10.1007/s41048-016-0018-3. Epub 2016 Mar 17.
Phenanthrene is one of the most recalcitrant components of crude oil-contaminated wastewater. An efficient phenanthrene-degrading bacterium sp. strain named LZ-Q was isolated from oil-contaminated soil near the sewage outlet of a petrochemical company. sp. LZ-Q is able to degrade 1000 mg/L phenanthrene in Bushnell-Hass mineral salt medium. It also degrades other polycyclic aromatic hydrocarbons such as naphthalene, anthracene, pyrene, petrol, and diesel at broad ranges of salinities of 5 g/L to 75 g/L, pHs of 5.0-10.0, and temperatures of 10-42 °C. Therefore, sp. LZ-Q could be a good candidate for remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated wastewater. A membrane bioreactor (MBR) was applied to investigate the remediation ability of the strain LZ-Q. Wastewater containing phenanthrene with pH of 8, salinity of 35 g/L, and COD of 500 mg/L was continuously added to the system (HRT = 3 h). Results showed that sp. LZ-Q is capable of degrading 96% of 20 mg/L phenanthrene and 94% of 500 mg/L COD for 60 days in a continuous mode. These results showed that the MBR system with strain LZ-Q might be a good approach for PAHs' remediation in industrial wastewaters.
菲是原油污染废水中最难降解的成分之一。从一家石化公司污水排放口附近的石油污染土壤中分离出一株高效降解菲的细菌菌株LZ-Q。菌株LZ-Q能够在布什内尔-哈斯矿物盐培养基中降解1000 mg/L的菲。它还能在5 g/L至75 g/L的宽盐度范围、5.0 - 10.0的pH范围以及10 - 42°C的温度下，降解萘、蒽、芘、汽油和柴油等其他多环芳烃。因此，菌株LZ-Q可能是修复多环芳烃（PAH）污染废水的良好候选菌株。应用膜生物反应器（MBR）研究菌株LZ-Q的修复能力。将pH为8、盐度为35 g/L、化学需氧量（COD）为500 mg/L的含菲废水连续添加到系统中（水力停留时间HRT = 3 h）。结果表明，菌株LZ-Q能够在连续模式下60天内降解96%的20 mg/L菲和94%的500 mg/L COD。这些结果表明，含有菌株LZ-Q的MBR系统可能是工业废水中PAHs修复的良好方法。
