Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Neuroimage. 2010 Apr 1;50(2):375-82. doi: 10.1016/j.neuroimage.2009.12.111. Epub 2010 Jan 7.
Green fluorescent protein (GFP) is a widely utilized molecular marker of gene expression. However, its use in in vivo imaging has been restricted to transparent tissue mainly due to the tissue penetrance limitation of optical imaging. Here, we report a novel approach to detect GFP with Magnetization transfer contrast (MTC) magnetic resonance imaging (MRI). MTC is an MRI methodology currently utilized to detect macromolecule changes such as decrease in myelin and increase in collagen content. We employed MTC MRI imaging to detect GFP both in vitro and in in vivo mouse models. We demonstrated that our approach produces values that are protein specific, and concentration dependent. This approach provides a flexible, non-invasive in vivo molecular MRI imaging strategy that is dependent upon the presence and concentration of the GFP reporter.
绿色荧光蛋白（GFP）是一种广泛应用于基因表达的分子标记物。然而，由于光学成像的组织穿透限制，其在体内成像中的应用主要局限于透明组织。在这里，我们报告了一种利用磁化传递对比（MTC）磁共振成像（MRI）检测 GFP 的新方法。MTC 是一种目前用于检测大分子变化的 MRI 方法，如髓鞘减少和胶原蛋白含量增加。我们采用 MTC MRI 成像技术在体外和体内小鼠模型中检测 GFP。我们证明了我们的方法产生的结果是蛋白质特异性的，并且与浓度有关。这种方法提供了一种灵活的、非侵入性的体内分子 MRI 成像策略，该策略依赖于 GFP 报告基因的存在和浓度。
